NF-kB p65 Kit ELISA

N° du produit ABIN7830876

Le kit ELISA NF-kB p65 (ABIN7830876) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain NF-kB p65.

Aperçu rapide pour NF-kB p65 Kit ELISA (ABIN7830876)

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit NF-kB p65 Kit ELISA

Fonction: RELA (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of RELA expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Autre désignation: RELA

Sujet:

Synonyms: MGC131774|NFKB3|p65

Gene Description: v-rel reticuloendotheliosis viral oncogene homolog A (avian)

ID gène: 5970

Pathways: Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins