Src Kit ELISA

N° du produit ABIN7830896

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain Src.

Aperçu rapide pour Src Kit ELISA (ABIN7830896)

Antigène: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit Src Kit ELISA

Fonction: SRC (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of SRC expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Autre désignation: SRC

Sujet:

Synonyms: ASV|SRC1|c-SRC|p60-Src

Gene Description: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

ID gène: 6714

Pathways: Signalistation JAK/STAT, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling