HRAS Kit ELISA

N° du produit ABIN7830972

Le kit ELISA HRAS (ABIN7830972) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain HRAS.

Aperçu rapide pour HRAS Kit ELISA (ABIN7830972)

Antigène: HRAS (HRas proto-oncogene, GTPase (HRAS))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Adherent Cell Culture, Cell Culture Cells, Cell Samples

Détail du produit HRAS Kit ELISA

Fonction: HRAS/KRAS/NRAS (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of HRAS/KRAS/NRAS expression in cultured cells.

Analytical Method: Qualitative

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: HRAS (HRas proto-oncogene, GTPase (HRAS))

Autre désignation: HRAS

Sujet:

Synonyms: C-BAS/HAS|C-H-RAS|C-HA-RAS1|CTLO|H-RASIDX|HAMSV|HRAS1|K-RAS|N-RAS|RASH1

Gene Description: v-Ha-ras Harvey rat sarcoma viral oncogene homolog

ID gène: 3265

Pathways: Signalisation p53, Signalisation MAPK, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling