NF-kB p65 Kit ELISA

N° du produit ABIN8126254

Le kit ELISA NF-kB p65 (ABIN8126254) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain NF-kB p65.

Aperçu rapide pour NF-kB p65 Kit ELISA (ABIN8126254)

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.313 ng/mL - 20 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit NF-kB p65 Kit ELISA

Seuil minimal de détection: 0.313 ng/mL

Fonction: This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytical Method: Quantitative

Sensibilité: 0.188 ng/mL

Attributs du produit: This immunoassay kit allows for the in vitro quantitative determination of Human NF-kB p65 concentrations in serum, plasma. It also detects cytoplasmic, nuclear and total NF-kB p65 from cells. Detection Range: 0.313 - 20 ng/mL Sensitivity: < 0.188 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human p65 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human p65 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human p65 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human p65 can be calculated using the standard curve. Kit Components

Restrictions: For Research Use only

Stockage

Stockage commentaire: Please refer to the manual

Détail du antigène

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Autre désignation: NF-kB p65 (Nuclear Factor Kappa B p65)

Pathways: Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins