Complement Factor H Kit ELISA

N° du produit ABIN955832

Détail du produit Complement Factor H Kit ELISA

Antigène: Complement Factor H (CFH)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 ng/mL

Seuil minimal de détection: 15.6 ng/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of mouse CF-H in serum, plasma and other biological fluids.

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of mouse CF-H. No significant cross- reactivity or interference was observed.

Sensibilité: The minimum detectable dose of mouse CF-H is typically less than 6.7 ng/mL.
The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero calibrator plus three standard deviations.

Attributs du produit: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CF-H. Calibrators or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CF-H. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CF-H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CF-H in the samples is then determined by comparing the O.D. of the samples to the calibration curve.

Ingrédients: Pre-coated, ready to use 96-well strip plate (1x)
Calibrator (lyophilized) (2x)
Calibrator Diluent (1 x 20 mL)
Detection Reagent A (1 x 120 µL)
Detection Reagent B (1 x 120 µL)
Assay Diluent A (2X concentrate) (1 x 6 mL)
Assay Diluent B (2X concentrate) (1 x 6 mL)
TMB Substrate (1 x 9 mL)
Stop Solution (1 x 6 mL)
Wash Buffer (30X concentrate) (1 x 20 mL)
Plate sealer for 96 wells (4x).

Matériel non inclus: 1. Microplate reader with 450 +/- 10 nm filter.
2. Precision single and multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. De-ionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Commentaires:

The calibration curve concentrations used for the ELISA's were 1000 ng/mL, 500 ng/mL, 250 ng/mL, 125 ng/mL, 62.5 ng/mL, 31.2 ng/mL, 15.6 ng/mL.

Plaque: Pre-coated

Calcul des résultats:

Average the duplicate readings for each calibrator, control, and samples and subtract the average zero calibrator optical density. Create a calibration curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a calibration curve by plotting the mean absorbance for each calibrator on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the CF-H concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. It is recommended to use some related software to do this calculation. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.

Date de péremption: The expiry date is stated on the label.

Détail du antigène

Antigène: Complement Factor H (CFH)

Autre désignation: Complement Factor H (CF-H) (CFH Produits)

Synonymes: AHUS1 Kit ELISA, AMBP1 Kit ELISA, ARMD4 Kit ELISA, ARMS1 Kit ELISA, CFHL3 Kit ELISA, FH Kit ELISA, FHL1 Kit ELISA, HF Kit ELISA, HF1 Kit ELISA, HF2 Kit ELISA, HUS Kit ELISA, Mud-1 Kit ELISA, NOM Kit ELISA, Sas-1 Kit ELISA, Sas1 Kit ELISA, Fh Kit ELISA, CFH Kit ELISA, im:7147412 Kit ELISA, si:ch211-207o17.2 Kit ELISA, fH Kit ELISA, armd4 Kit ELISA, arms1 Kit ELISA, cfh Kit ELISA, cfhl3 Kit ELISA, fhl1 Kit ELISA, hf1 Kit ELISA, hf2 Kit ELISA, hus Kit ELISA, complement factor H Kit ELISA, complement component factor h Kit ELISA, complement factor H-related protein 5 Kit ELISA, complement factor H L homeolog Kit ELISA, CFH Kit ELISA, Cfh Kit ELISA, cfh Kit ELISA, LOC100399888 Kit ELISA, LOC100733902 Kit ELISA, cfh.L Kit ELISA

Pathways: Système du Complément, Cellular Response to Molecule of Bacterial Origin