FEN1 Protein (AA 1-380)

N° du produit ABIN1690883

Détail du produit

Antigène: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-380

Origine: Humain

Source: Escherichia coli (E. coli)

Fonction: Recombinant Human Flap Endonuclease 1/FEN-1

Séquence: MGIQGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET TSHLMGMFYR TIRMMENGIK PVYVFDGKPP QLKSGELAKR SERRAEAEKQ LQQAQAAGAE QEVEKFTKRL VKVTKQHNDE CKHLLSLMGI PYLDAPSEAE ASCAALVKAG KVYAAATEDM DCLTFGSPVL MRHLTASEAK KLPIQEFHLS RILQELGLNQ EQFVDLCILL GSDYCESIRG IGPKRAVDLI QKHKSIEEIV RRLDPNKYPV PENWLHKEAH QLFLEPEVLD PESVELKWSE PNEEELIKFM CGEKQFSEER IRSGVKRLSK SRQGSTQGRL DDFFKVTGSL SSAKRKEPEP KGSTKKKAKT GAAGKFKRGK

Attributs du produit: Recombinant Human Flap Endonuclease 1/FEN-1 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Lys380) of Human FEN-1.

Pureté: > 95 % as determined by reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 50 mM Tris, 50 mM NaCl, 1 mM DDT, 10 % Glycerol, pH 8.0.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Stock: -80 °C

Stockage commentaire: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Autre désignation: flap-endonuclease-1 (FEN1 Produits)

Synonymes: FEN-1 Protein, FEN1 Protein, AW538437 Protein, MF1 Protein, RAD2 Protein, cb879 Protein, fen-1 Protein, fen1 Protein, mf1 Protein, rad2 Protein, xFEN1a Protein, flap structure-specific endonuclease 1 Protein, flap endonuclease 1-like Protein, Flap endonuclease-1 Protein, 5'-3' exonuclease family protein Protein, flap structure specific endonuclease 1 Protein, flap structure-specific endonuclease 1 L homeolog Protein, nuclease, Rad2 family Protein, FEN1 Protein, LOC100808044 Protein, Bm1_49605 Protein, AT5G26680 Protein, Fen1 Protein, fen1 Protein, fen1.L Protein

Sujet: Flap Endonuclease 1 (FEN1) is a member of the XPG/RAD2 endonuclease family. During DNA replication, FEN1 cleaves the 5'-overhanging flap structure and processes the 5' ends of Okazaki fragments for synthesis. FEN1 also exhibits RNase H activity by possessing 5'-3' exonuclease activity on gapped double-stranded or nicked DNA, FEN1 is involved in the long patch base excision repair (LP-BER) pathway, it can cleave within the apurinic/apyrimidinic (AP) site-terminated flap. FEN1 can prevent flaps from equilibrating into structures that lead to duplications and deletions. FEN1 is also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Alternative Names: Flap Endonuclease 1, FEN-1, DNase IV, Flap Structure-Specific Endonuclease 1, Maturation Factor 1, MF1, hFEN-1, FEN1, RAD2

Poids moléculaire: 42.59 kDa

UniProt: P39748

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA