Bcl-2 Protein (AA 1-239) (GST tag)
(1 reference)-
- Antigène Voir toutes Bcl-2 (BCL2) Protéines
- Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-239
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Origine
- Humain
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Source
- Wheat germ
- Purification/Conjugué
- Cette Bcl-2 protéine est marqué à la GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Fonction
- BCL2 (Human) Recombinant Protein (P01)
- Séquence
- MAHAGRTGYD NREIVMKYIH YKLSQRGYEW DAGDVGAAPP GAAPAPGIFS SQPGHTPHPA ASRDPVARTS PLQTPAAPGA AAGPALSPVP PVVHLTLRQA GDDFSRRYRR DFAEMSSQLH LTPFTARGRF ATVVEELFRD GVNWGRIVAF FEFGGVMCVE SVNREMSPLV DNIALWMTEY LNRHLHTWIQ DNGGWDAFVE LYGPSMRPLF DFSWLSLKTL LSLALVGACI TLGAYLGHK
- Attributs du produit
- Human BCL2 full-length ORF ( AAH27258.1, 1 a.a. - 239 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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B-Cell CLL/lymphoma 2 (BCL2) (AA 5-205) protein (His tag)
BCL2 Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
B-Cell CLL/lymphoma 2 (BCL2) (AA 1-211) protein (His-IF2DI Tag)BCL2 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
B-Cell CLL/lymphoma 2 (BCL2) (AA 140-239) protein (GST tag)BCL2 Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AA, AP
B-Cell CLL/lymphoma 2 (BCL2) (AA 1-236) protein (Strep Tag)Crystallography grade BCL2 Origine: Souris Hôte: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
B-Cell CLL/lymphoma 2 (BCL2) (AA 1-239) protein (Strep Tag)Crystallography grade BCL2 Origine: Humain Hôte: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Commentaires
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Conseil sur la manipulation
- Aliquot to avoid repeated freezing and thawing.
- Stock
- -80 °C
- Stockage commentaire
- Best use within three months from the date of receipt of this protein.
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: "Threonine 56 phosphorylation of Bcl-2 is required for LRRK2 G2019S-induced mitochondrial depolarization and autophagy." dans: Biochimica et biophysica acta, Vol. 1852, Issue 1, pp. 12-21, (2014) (PubMed).
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: "Threonine 56 phosphorylation of Bcl-2 is required for LRRK2 G2019S-induced mitochondrial depolarization and autophagy." dans: Biochimica et biophysica acta, Vol. 1852, Issue 1, pp. 12-21, (2014) (PubMed).
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- Antigène
- Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
- Autre désignation
- BCL2 (BCL2 Produits)
- Synonymes
- Bcl-2 Protein, PPP1R50 Protein, bcl-2 Protein, AW986256 Protein, C430015F12Rik Protein, D630044D05Rik Protein, D830018M01Rik Protein, BCL-2 Protein, Bcl2 Protein, BCL2, apoptosis regulator Protein, B cell leukemia/lymphoma 2 Protein, B-cell CLL/lymphoma 2a Protein, B-cell CLL/lymphoma 2 protein Protein, BCL2 Protein, Bcl2 Protein, bcl2a Protein, BCL-2 Protein
- Sujet
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Full Gene Name: B-cell CLL/lymphoma 2
Synonyms: Bcl-2 - ID gène
- 596
- Pathways
- Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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