IL1A
Origine: Rat
Hôte: Escherichia coli (E. coli)
Recombinant
>95 % as determined by reducing SDS-PAGE.
Indications d'application
Optimal working dilution should be determined by the investigator.,Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Prior to use, reconstitute the lyophilized powder with 0.2 mLof PBS containing a carrier protein (e.g., 1 % BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70 °C for up to one month.
Buffer
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1 % BSA.
Conseil sur la manipulation
Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Stock
4 °C
Stockage commentaire
Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt.
IL-1 was isolated from human blood that had been exposed to a pathogenic bacterium. IL-1 is a pyrogen, and it is an activating factor for lymphocytes. It also damaged joints and influenced liver proteins (2). IL-1α binds to the cell surface type I and II IL-1 receptors (IL-1RI and IL-1RII). IL-1 and -β and IL-1RA can compete for binding to these receptors. However, only IL-1RI, not IL-1RII, is functional because IL-1RII lacks a cytoplasmic domain and is thus unable to transmit signals to downstream steps (3). IL-1α is expressed by cancer cells and promotes angiogenesis and metastasis of pancreatic cancer and human gastric cancer cell lines (4). In addition, IL-1 induced the expression of VEGF in colon cancer (5).