DNMT3A Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)
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- Antigène Voir toutes DNMT3A Protéines
- DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
- Attributs du protein
- Transcript Variant 3
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Origine
- Humain
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette DNMT3A protéine est marqué à la Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
- Specificité
- Optimal preservation of protein structure, post-translational modifications and functions.
- Attributs du produit
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- Recombinant human DNMT3A (transcript variant 3) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Pureté
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Biological Activity Comment
- DNMT3A activity verified in a biochemical assay:,DNMT3A activity verified in a biochemical assay:,DNMT3A activity verified in a biochemical assay:
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- Indications d'application
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Commentaires
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- > 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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DNA G-quadruplexes show strong interaction with DNA methyltransferases in vitro." dans: FEBS letters, Vol. 590, Issue 17, pp. 2870-83, (2016) (PubMed).
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DNA G-quadruplexes show strong interaction with DNA methyltransferases in vitro." dans: FEBS letters, Vol. 590, Issue 17, pp. 2870-83, (2016) (PubMed).
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- Antigène
- DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))
- Autre désignation
- Dnmt3a (DNMT3A Produits)
- Synonymes
- DNMT3A Protein, DNMT3A2 Protein, M.HsaIIIA Protein, MmuIIIA Protein, dnmt3a1 Protein, dnmt6 Protein, DNA methyltransferase 3 alpha Protein, DNA methyltransferase 3A Protein, DNA (cytosine-5-)-methyltransferase 3 alpha b Protein, DNMT3A Protein, Dnmt3a Protein, dnmt3a Protein, dnmt3ab Protein
- Sujet
- CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.
- Poids moléculaire
- 101.7 kDa
- NCBI Accession
- NP_072046
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