DNMT3A Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)

N° du produit ABIN2719617

Détail du produit

Antigène: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: Transcript Variant 3

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette DNMT3A protéine est marqué à la Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)

Specificité: Optimal preservation of protein structure, post-translational modifications and functions.

Attributs du produit:

• Recombinant human DNMT3A (transcript variant 3) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Biological Activity Comment: DNMT3A activity verified in a biochemical assay:,DNMT3A activity verified in a biochemical assay:,DNMT3A activity verified in a biochemical assay:

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Référence

Cree, Fredericks, Miller, Pearce, Filichev, Fee, Kennedy: "DNA G-quadruplexes show strong interaction with DNA methyltransferases in vitro." dans: FEBS letters, Vol. 590, Issue 17, pp. 2870-83, (2016) (PubMed).

Détail du antigène

Antigène: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Autre désignation: Dnmt3a (DNMT3A Produits)

Synonymes: DNMT3A Protein, DNMT3A2 Protein, M.HsaIIIA Protein, MmuIIIA Protein, dnmt3a1 Protein, dnmt6 Protein, DNA methyltransferase 3 alpha Protein, DNA methyltransferase 3A Protein, DNA (cytosine-5-)-methyltransferase 3 alpha b Protein, DNMT3A Protein, Dnmt3a Protein, dnmt3a Protein, dnmt3ab Protein

Sujet: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.

Poids moléculaire: 101.7 kDa

NCBI Accession: NP_072046