TNFSF12
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by SDS-PAGE and Coomassie blue staining
Func, PI, AbP, STD
Active
TWEAK (TNFSF12)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))
Type de proteíne
Recombinant
Activité biologique
Active
Origine
Humain
Source
Escherichia coli (E. coli)
Application
Functional Studies (Func), Protein Interaction (PI), Antibody Production (AbP), Standard (STD)
Specificité
Optimal preservation of protein structure, post-translational modifications and functions.
Attributs du produit
Recombinant human TWEAK / TNFSF12 protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
Pureté
> 95 % as determined by SDS-PAGE and Coomassie blue staining
niveau d'endotoxine
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Biological Activity Comment
Assay #1: ED50 as determined by the dose-dependent stimulation of IL-8 production by Human PBMC is less than 10 ng/ml. Assay #2: TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN-gamma. ED50 for this effect is between 30-45 ng/ml.
TNFSF12
Origine: Humain
Hôte: CHO Cells
Recombinant
> 95 % as analyzed by SDS-PAGE and HPLC.
Active
Indications d'application
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
Restrictions
For Research Use only
Buffer
Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
Conseil sur la manipulation
Resuspend the protein in the desired concentration in proper buffer
Stock
-80 °C
Stockage commentaire
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Antigène
TWEAK (TNFSF12)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))
This gene encodes a member of the tumor necrosis factor superfamily. It encodes a hybrid protein composed of the cytoplasmic and transmembrane domains of family member 12 fused to the C-terminal domain of family member 13. The hybrid protein is membrane anchored and presents the receptor-binding domain of family member 13 at the cell surface. It stimulates cycling in T- and B-lymphoma cell lines.