VAV1 Protein (Myc-DYKDDDDK Tag)

N° du produit ABIN2735197

Détail du produit

Antigène: VAV1 (Vav 1 Oncogene (VAV1))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette VAV1 protéine est marqué à la Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Attributs du produit:

• Recombinant human VAV1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Référence

Mahankali, Peng, Henkels, Dinauer, Gomez-Cambronero: "Phospholipase D2 (PLD2) is a guanine nucleotide exchange factor (GEF) for the GTPase Rac2." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 49, pp. 19617-22, (2011) (PubMed).

Détail du antigène

Antigène: VAV1 (Vav 1 Oncogene (VAV1))

Autre désignation: Vav1 (VAV1 Produits)

Synonymes: VAV Protein, Vav Protein, vav-T Protein, vav guanine nucleotide exchange factor 1 Protein, vav 1 oncogene Protein, vav guanine nucleotide exchange factor 1 L homeolog Protein, VAV1 Protein, Vav1 Protein, vav1.L Protein

Sujet: This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Poids moléculaire: 98.1 kDa

NCBI Accession: NP_005419

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, BCR Signaling