TRKB Protein (AA 455-822) (His tag)

N° du produit ABIN3094259

Détail du produit

Antigène: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Type de proteíne: Recombinant

Attributs du protein: AA 455-822

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette TRKB protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), ELISA, Western Blotting (WB), Crystallization (Crys)

Séquence: KLARHSKFGM KGPASVISND DDSASPLHHI SNGSNTPSSS EGGPDAVIIG MTKIPVIENP QYFGITNSQL KPDTFVQHIK RHNIVLKREL GEGAFGKVFL AECYNLCPEQ DKILVAVKTL KDASDNARKD FHREAELLTN LQHEHIVKFY GVCVEGDPLI MVFEYMKHGD LNKFLRAHGP DAVLMAEGNP PTELTQSQML HIAQQIAAGM VYLASQHFVH RDLATRNCLV GENLLVKIGD FGMSRDVYST DYYRVGGHTM LPIRWMPPES IMYRKFTTES DVWSLGVVLW EIFTYGKQPW YQLSNNEVIE CITQGRVLQR PRTCPQEVYE LMLGCWQREP HMRKNIKGIH TLLQNLAKAS PVYLDILG
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human NTRK2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Autre désignation: NTRK2 (NTRK2 Produits)

Synonymes: trkb Protein, trkb-b Protein, xtrkb Protein, si:ch211-220b11.1 Protein, trkB2 Protein, NTRK2 Protein, ntrk2 Protein, gp145-trkb Protein, TRKB Protein, GP145-TrkB Protein, trk-B Protein, AI848316 Protein, C030027L06Rik Protein, Tkrb Protein, trkB Protein, RATTRKB1 Protein, TRKB1 Protein, Trk-B Protein, TrkB Protein, neurotrophic receptor tyrosine kinase 2 Protein, neurotrophic receptor tyrosine kinase 2 L homeolog Protein, neurotrophic tyrosine kinase, receptor, type 2b Protein, neurotrophic receptor tyrosine kinase 2 S homeolog Protein, neurotrophic tyrosine kinase, receptor, type 2 Protein, NTRK2 Protein, ntrk2.L Protein, ntrk2b Protein, ntrk2.S Protein, ntrk2 Protein, Ntrk2 Protein

Sujet: Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. {ECO:0000269|PubMed:15494731}.

Poids moléculaire: 42.5 kDa Including tag.

UniProt: Q16620

Pathways: Signalisation RTK, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport