LRP5 Protein (AA 32-1615) (rho-1D4 tag)

N° du produit ABIN3110628

Détail du produit

Antigène: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Type de proteíne: Recombinant

Attributs du protein: AA 32-1615

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette LRP5 protéine est marqué à la rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: SPLLLFANRR DVRLVDAGGV KLESTIVVSG LEDAAAVDFQ FSKGAVYWTD VSEEAIKQTY LNQTGAAVQN VVISGLVSPD GLACDWVGKK LYWTDSETNR IEVANLNGTS RKVLFWQDLD QPRAIALDPA HGYMYWTDWG ETPRIERAGM DGSTRKIIVD SDIYWPNGLT IDLEEQKLYW ADAKLSFIHR ANLDGSFRQK VVEGSLTHPF ALTLSGDTLY WTDWQTRSIH ACNKRTGGKR KEILSALYSP MDIQVLSQER QPFFHTRCEE DNGGCSHLCL LSPSEPFYTC ACPTGVQLQD NGRTCKAGAE EVLLLARRTD LRRISLDTPD FTDIVLQVDD IRHAIAIDYD PLEGYVYWTD DEVRAIRRAY LDGSGAQTLV NTEINDPDGI AVDWVARNLY WTDTGTDRIE VTRLNGTSRK ILVSEDLDEP RAIALHPVMG LMYWTDWGEN PKIECANLDG QERRVLVNAS LGWPNGLALD LQEGKLYWGD AKTDKIEVIN VDGTKRRTLL EDKLPHIFGF TLLGDFIYWT DWQRRSIERV HKVKASRDVI IDQLPDLMGL KAVNVAKVVG TNPCADRNGG CSHLCFFTPH ATRCGCPIGL ELLSDMKTCI VPEAFLVFTS RAAIHRISLE TNNNDVAIPL TGVKEASALD FDVSNNHIYW TDVSLKTISR AFMNGSSVEH VVEFGLDYPE GMAVDWMGKN LYWADTGTNR IEVARLDGQF RQVLVWRDLD NPRSLALDPT KGYIYWTEWG GKPRIVRAFM DGTNCMTLVD KVGRANDLTI DYADQRLYWT DLDTNMIESS NMLGQERVVI ADDLPHPFGL TQYSDYIYWT DWNLHSIERA DKTSGRNRTL IQGHLDFVMD ILVFHSSRQD GLNDCMHNNG QCGQLCLAIP GGHRCGCASH YTLDPSSRNC SPPTTFLLFS QKSAISRMIP DDQHSPDLIL PLHGLRNVKA IDYDPLDKFI YWVDGRQNIK RAKDDGTQPF VLTSLSQGQN PDRQPHDLSI DIYSRTLFWT CEATNTINVH RLSGEAMGVV LRGDRDKPRA IVVNAERGYL YFTNMQDRAA KIERAALDGT EREVLFTTGL IRPVALVVDN TLGKLFWVDA DLKRIESCDL SGANRLTLED ANIVQPLGLT ILGKHLYWID RQQQMIERVE KTTGDKRTRI QGRVAHLTGI HAVEEVSLEE FSAHPCARDN GGCSHICIAK GDGTPRCSCP VHLVLLQNLL TCGEPPTCSP DQFACATGEI DCIPGAWRCD GFPECDDQSD EEGCPVCSAA QFPCARGQCV DLRLRCDGEA DCQDRSDEAD CDAICLPNQF RCASGQCVLI KQQCDSFPDC IDGSDELMCE ITKPPSDDSP AHSSAIGPVI GIILSLFVMG GVYFVCQRVV CQRYAGANGP FPHEYVSGTP HVPLNFIAPG GSQHGPFTGI ACGKSMMSSV SLMGGRGGVP LYDRNHVTGA SSSSSSSTKA TLYPPILNPP PSPATDPSLY NMDMFYSSNI PATARPYRPY IIRGMAPPTT PCSTDVCDSD YSASRWKASK YYLDLNSDSD PYPPPPTPHS QYLSAEDSCP PSPATERSYF HLFPPPPSPC TDSS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human LRP5 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin-free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Autre désignation: LRP5 (LRP5 Produits)

Synonymes: Xlrp5 Protein, bmnd1 Protein, evr1 Protein, evr4 Protein, hbm Protein, lr3 Protein, lrp5-A Protein, lrp7 Protein, oppg Protein, ops Protein, opta1 Protein, vbch2 Protein, LRP5 Protein, LRP6 Protein, BMND1 Protein, EVR1 Protein, EVR4 Protein, HBM Protein, LR3 Protein, LRP7 Protein, OPPG Protein, OPS Protein, OPTA1 Protein, VBCH2 Protein, mKIAA4142 Protein, low-density lipoprotein receptor-related protein 5 Protein, LDL receptor related protein 5 Protein, LDL receptor related protein 5 L homeolog Protein, low density lipoprotein receptor-related protein 5 Protein, LDL receptor related protein 5 like Protein, LOC100147353 Protein, LRP5 Protein, lrp5.L Protein, lrp5 Protein, Lrp5 Protein, LRP5L Protein

Sujet: Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Appears be required for postnatal control of vascular regression in the eye. Required for posterior patterning of the epiblast during gastrulation. {ECO:0000269|PubMed:11336703, ECO:0000269|PubMed:11448771, ECO:0000269|PubMed:14727154, ECO:0000269|PubMed:14731402, ECO:0000269|PubMed:15778503}.

Poids moléculaire: 177.2 kDa Including tag.

UniProt: O75197

Pathways: Signalisation WNT, Stem Cell Maintenance, Positive Regulation of fat Cell Differentiation