LRP5
(Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))
Type de proteíne
Recombinant
Attributs du protein
AA 644-1263
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette LRP5 protéine est marqué à la mFc Tag.
Fonction
Human LRP-5 Protein
Séquence
Glu644-Gln1263
Attributs du produit
Recombinant Human LRP-5 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Glu644-Gln1263.
Pureté
> 95 % as determined by Tris-Bis PAGE
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human DKK1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 0.20μg/ml determined by ELISA. See testing image for detail.
LRP5
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
> 90 %
WB, SDS, Imm, PC
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Date de péremption
12 months
Antigène
LRP5
(Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))
Xlrp5 Protein, bmnd1 Protein, evr1 Protein, evr4 Protein, hbm Protein, lr3 Protein, lrp5-A Protein, lrp7 Protein, oppg Protein, ops Protein, opta1 Protein, vbch2 Protein, LRP5 Protein, LRP6 Protein, BMND1 Protein, EVR1 Protein, EVR4 Protein, HBM Protein, LR3 Protein, LRP7 Protein, OPPG Protein, OPS Protein, OPTA1 Protein, VBCH2 Protein, mKIAA4142 Protein, low-density lipoprotein receptor-related protein 5 Protein, LDL receptor related protein 5 Protein, LDL receptor related protein 5 L homeolog Protein, low density lipoprotein receptor-related protein 5 Protein, LDL receptor related protein 5 like Protein, LOC100147353 Protein, LRP5 Protein, lrp5.L Protein, lrp5 Protein, Lrp5 Protein, LRP5L Protein
Sujet
A role for low-density lipoprotein-related receptor 5 (LRP5) in human bone was first established by the identification of genetic alterations that led to dramatic changes in bone mass. Shortly thereafter, mutations that altered the function of the sclerostin (SOST) gene were also associated with altered human bone mass. Subsequent studies of LRP5 and sclerostin have provided important insights into the mechanisms by which these proteins regulate skeletal homeostasis.
Poids moléculaire
96 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Tris-Bis PAGE result.