PAG1 Protein (AA 39-429) (His tag)

N° du produit ABIN3121921

Détail du produit

Antigène: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Type de proteíne: Recombinant

Attributs du protein: AA 39-429

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette PAG1 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Séquence: CSTCDREKKP RQHSGDHENL MNVPSDKDMF SHSATSLTTD ALASSEQNGV LTNGDILSED STLTCMQHYE EVQTSASDLL DSQDSTGKAK CHQSRELPRI PPENAVDEIL TARAADTELG PGVEGPYEVL KDSSSQENMV EDCLYETVKE IKEVADKGQG GKSKSTSALK ELQGAPMEGK ADFAEYASVD RNKKCRHSAN AESILGTCSD LDEESPPPVP VKLLDENANL PQEGGGQAEE QAAEGTGGHS KRFSSLSYKS REEDPTLTEE EISAMYSSVN KPGQSAHKPG PCMKGPESAC HSMKGLPQRS SSSCNDLYAT VKDFEKTPNS ISTLPPARRP SEEPEPDYEA IQTLNREDEK VPLETNGHHV PKESDYESIG DLQQCRDVTR L
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Pag1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Autre désignation: Pag1 (PAG1 Produits)

Synonymes: Cbp Protein, F730007C19Rik Protein, Pag Protein, CBP Protein, PAG Protein, MGC80192 Protein, phosphoprotein associated with glycosphingolipid microdomains 1 Protein, phosphoprotein membrane anchor with glycosphingolipid microdomains 1 Protein, phosphoprotein membrane anchor with glycosphingolipid microdomains 1 L homeolog Protein, Pag1 Protein, PAG1 Protein, pag1.L Protein, pag1 Protein

Sujet: Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling. {ECO:0000269|PubMed:12218089, ECO:0000269|PubMed:12612075, ECO:0000269|PubMed:14645715, ECO:0000269|PubMed:16166631}.

Poids moléculaire: 43.5 kDa Including tag.

UniProt: Q3U1F9

Pathways: Signalisation p53, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events