AP2A2 Protein (AA 2-938) (His tag)

N° du produit ABIN3133147

Détail du produit

Antigène: AP2A2 (Adaptor-Related Protein Complex 2, alpha 2 Subunit (AP2A2))

Type de proteíne: Recombinant

Attributs du protein: AA 2-938

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette AP2A2 protéine est marqué à la His tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: PAVSKGDGMR GLAVFISDIR NCKSKEAEIK RINKELANIR SKFKGDKALD GYSKKKYVCK LLFIFLLGHD IDFGHMEAVN LLSSNRYTEK QIGYLFISVL VNSNSELIRL INNAIKNDLA SRNPTFMGLA LHCIANVGSR EMAEAFAGEI PKILVAGDTM DSVKQSAALC LLRLYRTSPD LVPMGDWTSR VVHLLNDQHL GVVTAATSLI TTLAQKNPEE FKTSVSLAVS RLSRIVTSAS TDLQDYTYYF VPAPWLSVKL LRLLQCYPPP DPAVRGRLTE CLETILNKAQ EPPKSKKVQH SNAKNAVLFE AISLIIHHDS EPNLLVRACN QLGQFLQHRE TNLRYLALES MCTLASSEFS HEAVKTHIET VINALKTERD VSVRQRAVDL LYAMCDRSNA QQIVAEMLSY LETADYSIRE EIVLKVAILA EKYAVDYTWY VDTILNLIRI AGDYVSEEVW YRVIQIVINR DDVQGYAAKT VFEALQAPAC HENLVKVGGY ILGEFGNLIA GDPRSSPLIQ FNLLHSKFHL CSVPTRALLL STYIKFVNLF PEVKATIQDV LRSDSQLKNA DVELQQRAVE YLRLSTVAST DILATVLEEM PPFPERESSI LAKLKKKKGP STVTDLEETK RERSIDVNGG PEPVPASTSA ASTPSPSADL LGLGAVPPAP TGPPPSSGGG LLVDVFSDSA SAVAPLAPGS EDNFARFVCK NNGVLFENQL LQIGLKSEFR QNLGRMFIFY GNKTSTQFLN FTPTLICADD LQTNLNLQTK PVDPTVDGGA QVQQVVNIEC ISDFTEAPVL NIQFRYGGTF QNVSVKLPIT LNKFFQPTEM ASQDFFQRWK QLSNPQQEVQ NIFKAKHPMD TEITKAKIIG FGSALLEEVD PNPANFVGAG IIHTKTTQIG CLLRLEPNLQ AQMYRLTLRT SKDTVSQRLC ELLSEQF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Ap2a2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: AP2A2 (Adaptor-Related Protein Complex 2, alpha 2 Subunit (AP2A2))

Autre désignation: Ap2a2 (AP2A2 Produits)

Synonymes: MGC99219 Protein, ADTAB Protein, CLAPA2 Protein, HIP-9 Protein, HIP9 Protein, HYPJ Protein, 2410074K14Rik Protein, AF006990 Protein, AW146353 Protein, Adtab Protein, C78001 Protein, L25 Protein, adaptor related protein complex 2 alpha 2 subunit Protein, adaptor related protein complex 2 alpha 2 subunit L homeolog Protein, adaptor-related protein complex 2, alpha 2 subunit Protein, AP2A2 Protein, ap2a2.L Protein, Ap2a2 Protein

Sujet: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. {ECO:0000269|PubMed:10459011, ECO:0000269|PubMed:14745134, ECO:0000269|PubMed:15473838}.

Poids moléculaire: 104.8 kDa Including tag.

UniProt: P17427

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation, SARS-CoV-2 Protein Interactome