MCM3 Protein (AA 2-812) (His tag)

N° du produit ABIN3133430

Détail du produit

Antigène: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Type de proteíne: Recombinant

Attributs du protein: AA 2-812

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette MCM3 protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: AGTVVLDDVE LREAQRDYLD FLDDEEDQGI YQNKVRELIS DNQYRLIVSV NDLRRKNEKR ANRLLNNAFE ELVAFQRALK DFVASIDATY AKQYEEFYIG LEGSFGSKHV SPRTLTSCFL SCVVCVEGIV TKCSLVRPKV VRSVHYCPAT KKTIERRYSD LTTLVAFPSS SVYPTKDEEN NPLETEYGLS VYKDHQTITI QEMPEKAPAG QLPRSVDVIL DDDLVDKVKP GDRIQVVGTY RCLPGKKGCY TSGTFRTVLI ACNVKQMSKD IQPAFSADDI AKIKKFSKTR SKDVFEQLAR SLAPSIHGHD YVKKAILCLL LGGVERELEN GSHIRGDINI LLIGDPSVAK SQLLRYVLCT APRAIPTTGR GSSGVGLTAA VTTDQETGER RLEAGAMVLA DRGVVCIDEF DKMSDMDRTA IHEVMEQGRV TIAKAGIHAR LNARCSVLAA ANPVYGRYDQ YKTPMENIGL QDSLLSRFDL LFIMLDQMDP EQDREISDHV LRMHQYRAPG EQDGDALPLG SSVDILATDD PDFTQDDQQD TRIYEKHDSL LHGTKKKKEK MVSAAFMKKY IHVAKIIKPT LTQESAAYIA EEYSRLRSQD SMSSDTARTS PVTARTLETL IRLATAHAKA RMSKTVDLQD AEEAVELVQY AYFKKVLEKE KKRKKASEDE SDLEDEEEKS QEDTEQKRKR RKTHAKDGES YDPYDFSEAE TQMPQVHTPK TDDSQEKTDD SQETQDSQKV ELSEPRLKAF KAALLEVFQE AHEQSVGMLH LTESINRNRE EPFSSEEIQA CLSRMQDDNQ VMVSEGIVFL I
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Mcm3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Autre désignation: Mcm3 (MCM3 Produits)

Synonymes: HCC5 Protein, P1-MCM3 Protein, P1.h Protein, RLFB Protein, AL033361 Protein, C80350 Protein, Mcmd Protein, P1 Protein, p1.m Protein, cb32 Protein, chunp6867 Protein, wu:fa26g03 Protein, CG4206 Protein, DmMCM3 Protein, DmMcm3 Protein, DmeMCM3 Protein, Dmel\\CG4206 Protein, MCM3 Protein, McM3 Protein, Mcp-PCR2 Protein, PCR2 Protein, dMCM3 Protein, xmcm3 Protein, minichromosome maintenance complex component 3 Protein, MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) Protein, Minichromosome maintenance 3 Protein, minichromosome maintenance complex component 3 L homeolog Protein, MCM complex subunit Mcm3 Protein, MCM3 Protein, mcm3 Protein, Mcm3 Protein, mcm3.L Protein

Sujet: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.

Poids moléculaire: 92.4 kDa Including tag.

UniProt: P25206

Pathways: Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA