Notch1 Protein (AA 1747-2531) (His tag)

N° du produit ABIN3134777

Détail du produit

Antigène: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Type de proteíne: Recombinant

Attributs du protein: AA 1747-2531

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette Notch1 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Séquence: SRKRRRQHGQ LWFPEGFKVS EASKKKRREP LGEDSVGLKP LKNASDGALM DDNQNEWGDE DLETKKFRFE EPVVLPDLSD QTDHRQWTQQ HLDAADLRMS AMAPTPPQGE VDADCMDVNV RGPDGFTPLM IASCSGGGLE TGNSEEEEDA PAVISDFIYQ GASLHNQTDR TGETALHLAA RYSRSDAAKR LLEASADANI QDNMGRTPLH AAVSADAQGV FQILLRNRAT DLDARMHDGT TPLILAARLA VEGMLEDLIN SHADVNAVDD LGKSALHWAA AVNNVDAAVV LLKNGANKDM QNNKEETPLF LAAREGSYET AKVLLDHFAN RDITDHMDRL PRDIAQERMH HDIVRLLDEY NLVRSPQLHG TALGGTPTLS PTLCSPNGYL GNLKSATQGK KARKPSTKGL ACGSKEAKDL KARRKKSQDG KGCLLDSSSM LSPVDSLESP HGYLSDVASP PLLPSPFQQS PSMPLSHLPG MPDTHLGISH LNVAAKPEMA ALAGGSRLAF EPPPPRLSHL PVASSASTVL STNGTGAMNF TVGAPASLNG QCEWLPRLQN GMVPSQYNPL RPGVTPGTLS TQAAGLQHSM MGPLHSSLST NTLSPIIYQG LPNTRLATQP HLVQTQQVQP QNLQLQPQNL QPPSQPHLSV SSAANGHLGR SFLSGEPSQA DVQPLGPSSL PVHTILPQES QALPTSLPSS MVPPMTTTQF LTPPSQHSYS SSPVDNTPSH QLQVPEHPFL TPSPESPDQW SSSSPHSNIS DWSEGISSPP TTMPSQITHI PEAFK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Notch1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Autre désignation: Notch1 (NOTCH1 Produits)

Synonymes: notch1 Protein, NOTCH1 Protein, hn1 Protein, tan1 Protein, notch Protein, xotch Protein, xnotch Protein, notch-1 Protein, xnotch1 Protein, TAN1 Protein, hN1 Protein, 9930111A19Rik Protein, Mis6 Protein, N1 Protein, Tan1 Protein, lin-12 Protein, NOTCH Protein, Xotch Protein, notch1-a Protein, notch 1 Protein, fibropellin-1 Protein, Notch homolog 1, translocation-associated (Drosophila) Protein, Notch homolog 1, translocation-associated Protein, notch 1 S homeolog Protein, notch1 Protein, LOC100631526 Protein, NOTCH1 Protein, Notch1 Protein, notch1.S Protein

Sujet: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis, negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO). {ECO:0000269|PubMed:15965470, ECO:0000269|PubMed:18299578, ECO:0000269|PubMed:23160044, ECO:0000269|PubMed:23615612}.

Poids moléculaire: 85.4 kDa Including tag.

UniProt: Q01705

Pathways: Signalisation Notch, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development