Notch1 Protein (AA 19-526) (His-Avi Tag)

N° du produit ABIN7275363

Détail du produit

Antigène: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Type de proteíne: Recombinant

Attributs du protein: AA 19-526

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette Notch1 protéine est marqué à la His-Avi Tag.

Fonction: Human Notch 1 Protein

Séquence: Ala19-Gln526

Attributs du produit: Recombinant Human Notch 1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala19-Gln526.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 0.48 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Autre désignation: Notch 1 (NOTCH1 Produits)

Synonymes: notch1 Protein, NOTCH1 Protein, hn1 Protein, tan1 Protein, notch Protein, xotch Protein, xnotch Protein, notch-1 Protein, xnotch1 Protein, TAN1 Protein, hN1 Protein, 9930111A19Rik Protein, Mis6 Protein, N1 Protein, Tan1 Protein, lin-12 Protein, NOTCH Protein, Xotch Protein, notch1-a Protein, notch 1 Protein, fibropellin-1 Protein, Notch homolog 1, translocation-associated (Drosophila) Protein, Notch homolog 1, translocation-associated Protein, notch 1 S homeolog Protein, notch1 Protein, LOC100631526 Protein, NOTCH1 Protein, Notch1 Protein, notch1.S Protein

Sujet: Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes.Notch-1 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Poids moléculaire: 56.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

UniProt: P46531

Pathways: Signalisation Notch, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development