EPH Receptor B2 Protein (EPHB2) (AA 19-986) (rho-1D4 tag)

N° du produit ABIN3134501

Détail du produit

Antigène: EPH Receptor B2 (EPHB2)

Type de proteíne: Recombinant

Attributs du protein: AA 19-986

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette EPH Receptor B2 protéine est marqué à la rho-1D4 tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Séquence: VEETLMDSTT ATAELGWMVH PPSGWEEVSG YDENMNTIRT YQVCNVFESS QNNWLRTKFI RRRGAHRIHV EMKFSVRDCS SIPSVPGSCK ETFNLYYYEA DFDLATKTFP NWMENPWVKV DTIAADESFS QVDLGGRVMK INTEVRSFGP VSRNGFYLAF QDYGGCMSLI AVRVFYRKCP RIIQNGAIFQ ETLSGAESTS LVAARGSCIA NAEEVDVPIK LYCNGDGEWL VPIGRCMCKA GFEAVENGTV CRGCPSGTFK ANQGDEACTH CPINSRTTSE GATNCVCRNG YYRADLDPLD MPCTTIPSAP QAVISSVNET SLMLEWTPPR DSGGREDLVY NIICKSCGSG RGACTRCGDN VQYAPRQLGL TEPRIYISDL LAHTQYTFEI QAVNGVTDQS PFSPQFASVN ITTNQAAPSA VSIMHQVSRT VDSITLSWSQ PDQPNGVILD YELQYYEKEL SEYNATAIKS PTNTVTVQGL KAGAIYVFQV RARTVAGYGR YSGKMYFQTM TEAEYQTSIK EKLPLIVGSS AAGLVFLIAV VVIAIVCNRR GFERADSEYT DKLQHYTSGH MTPGMKIYID PFTYEDPNEA VREFAKEIDI SCVKIEQVIG AGEFGEVCSG HLKLPGKREI FVAIKTLKSG YTEKQRRDFL SEASIMGQFD HPNVIHLEGV VTKSTPVMII TEFMENGSLD SFLRQNDGQF TVIQLVGMLR GIAAGMKYLA DMNYVHRDLA ARNILVNSNL VCKVSDFGLS RFLEDDTSDP TYTSALGGKI PIRWTAPEAI QYRKFTSASD VWSYGIVMWE VMSYGERPYW DMTNQDVINA IEQDYRLPPP MDCPSALHQL MLDCWQKDRN HRPKFGQIVN TLDKMIRNPN SLKAMAPLSS GINLPLLDRT IPDYTSFNTV DEWLEAIKMG QYKESFANAG FTSFDVVSQM MMEDILRVGV TLAGHQKKIL NSIQVMRAQM NQIQSVEV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Ephb2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin-free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: EPH Receptor B2 (EPHB2)

Autre désignation: Ephb2 (EPHB2 Produits)

Synonymes: CAPB Protein, DRT Protein, EK5 Protein, EPHT3 Protein, ERK Protein, Hek5 Protein, PCBC Protein, Tyro5 Protein, ERK2 Protein, ERT1 Protein, MAPK2 Protein, P42MAPK Protein, PRKM1 Protein, PRKM2 Protein, p38 Protein, p40 Protein, p41 Protein, p41mapk Protein, RGD1564232 Protein, Cek5 Protein, Drt Protein, ETECK Protein, Erk Protein, Nuk Protein, Prkm5 Protein, Qek5 Protein, Sek3 Protein, cek5 Protein, ephb2 Protein, xEphB2 Protein, si:ch73-160e9.1 Protein, tpx2 Protein, wu:fb09e10 Protein, wu:fc64a03 Protein, wu:fi18g12 Protein, zgc:112296 Protein, EPH receptor B2 Protein, mitogen-activated protein kinase 1 Protein, Eph receptor B2 Protein, EPH receptor B2 L homeolog Protein, eph receptor B2a Protein, eph receptor B2b Protein, EPHB2 Protein, MAPK1 Protein, Ephb2 Protein, ephb2.L Protein, ephb2a Protein, ephb2b Protein

Sujet: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor. {ECO:0000269|PubMed:10839360, ECO:0000269|PubMed:14691139, ECO:0000269|PubMed:15223334, ECO:0000269|PubMed:21029865, ECO:0000269|PubMed:8689685, ECO:0000269|PubMed:8947026, ECO:0000269|PubMed:9990854}.

Poids moléculaire: 109.2 kDa Including tag.

UniProt: P54763

Pathways: Signalisation RTK, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins