MYD88 Protein (AA 148-296)

N° du produit ABIN4886401

Détail du produit

Antigène: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Type de proteíne: Recombinant

Attributs du protein: AA 148-296

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys), Functional Studies (Func)

Séquence: TL DDPLGHMPER FDAFICYCPS DIQFVQEMIR QLEQTNYRLK LCVSDRDVLP GTCVWSIASE LIEKRCRRMV VVVSDDYLQS KECDFQTKFA LSLSPGAHQK RLIPIKYKAM KKEFPSILRF ITVCDYTNPC TKSWFWTRLA KALSLP
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human MYD88 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

The protein will be expressed with a N-terminal GST-tag. After a GST purification, the GST tag is cleaved off and the domain is purified by a gel filtration column. Because the GST and the Tir domain probably cannot be separated because of their size, an additionally cation exchange chromatography has to be carried out.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Autre désignation: MYD88 (MYD88 Produits)

Synonymes: MYD88D Protein, XMyD88 Protein, myd88d Protein, MGC84928 Protein, CG2078 Protein, DMMYD88 Protein, DmMyD88 Protein, DmMyd88 Protein, Dmel\\CG2078 Protein, EP(2)2535 Protein, Kra Protein, LD20892 Protein, MYD88 Protein, MyD88 Protein, Myd88F Protein, dMyD88 Protein, dMyd88 Protein, kra Protein, myd88 Protein, zgc:103541 Protein, GB12344 Protein, NV10640 Protein, myd88-a Protein, myd88-b Protein, myeloid differentiation primary response 88 Protein, myeloid differentiation primary response 88 S homeolog Protein, myeloid differentiation primary response gene 88 Protein, CG2078 gene product from transcript CG2078-RB Protein, myeloid differentiation primary response protein MyD88-A Protein, myeloid differentiation primary response protein MyD88 Protein, myeloid differentiation factor 88 Protein, myeloid differentiation primary response 88 L homeolog Protein, MYD88 Protein, myd88.S Protein, Myd88 Protein, myd88 Protein, LOC413194 Protein, LOC100118553 Protein, LOC575153 Protein, myd88.L Protein

Sujet: Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine. {ECO:0000269|PubMed:15361868, ECO:0000269|PubMed:18292575, ECO:0000269|PubMed:19506249, ECO:0000269|PubMed:24316379, ECO:0000269|PubMed:9013863}.

UniProt: Q99836

Pathways: Signalisation NF-kappaB, Signalisation TLR, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades