BDNF
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
Greater than 95 % as determined by SEC-HPLC and reducing SDS-PAGE.
SDS, WB, ELISA, MS
SDS-PAGE (SDS), Western Blotting (WB), ELISA, Mass Spectrometry (MS)
Fonction
Recombinant Human Pro-BDNF Protein is produced by E. coli. It is a single non-glycosylated polypeptide chain containing 2 X 229 amino acids as a homodimer with a molecular mass of 52 kD.
BDNF
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
>85 % as determined by reducing SDS-PAGE.
Restrictions
For Research Use only
Format
Lyophilized
Buffer
Lyophilized from a 0.2 μM filtered solution of 20 mM PB, 250 mM NaCl, pH 7.2.
Conseil sur la manipulation
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Stock
-20 °C
Stockage commentaire
Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.