WNT3A
(Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))
Type de proteíne
Recombinant
Activité biologique
Active
Attributs du protein
AA 19-352
Origine
Humain
Source
Mammalian Cells
Application
Functional Studies (Func)
Specificité
Stem cell or organoid culture
Purification
This protein was purified using a combination of ion exchange, affinity column with Wnt signaling inhibitor-bound sepharose beads, and followed by gel filtration.
Pureté
90-95 %
niveau d'endotoxine
Less than 0.1 ng/μg (1 IEU/μg)
Biological Activity Comment
Wnt3a activity has been measured using TCF-based Wnt reporter stable cell line. 10 ng/mL of Wnt3a (Lot: 17Feb2016) generate 50- fold increase of luciferase activity compared to control (buffer without Wnt3a).
30-60 ng/mL To treat cell lines, dilute the protein solution at least 200 times in medium, to treat stem cells, dilute the protein solution at least 500 times in medium. Diluted Wnt proteins in medium or phosphate buffer can be stored at 4 °C for few days only.
Restrictions
For Research Use only
Format
Liquid
Buffer
Phosphate buffer pH 7.4-7.6, 1 % CHAPS, 0.1 % BSA
Conseil sur la manipulation
Mix the protein by pipetting up and down but not by vortexing
Stock
-20 °C
Stockage commentaire
Keep the protein frozen until use. Refreeze aliquots at -20C or below but avoid freeze-thaw circles.
Antigène
WNT3A
(Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))
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Sujet
Protein Wnt-3a is a protein that is encoded by the WNT3A gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Human Wnt3a shows 96 % amino acid identity to mouse Wnt3a protein
Poids moléculaire
41 kDa evaluated by SDS-PAGE under reducing conditions