SMUG1 Protein (AA 1-177, full length) (His-SUMO Tag)

N° du produit ABIN5711385

Détail du produit

Antigène: SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))

Type de proteíne: Recombinant

Attributs du protein: full length, AA 1-177

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette SMUG1 protéine est marqué à la His-SUMO Tag.

Application: SDS-PAGE (SDS)

Séquence: MPQAFLLGSI HEPAGALMEP QPCPGSLAES FLEEELRLNA ELSQLQFSEP VGIIYNPVEY AWEPHRNYVT RYCQGPKEVL FLGMNPGPFG MAQTGVPFGE VSMVRDWLGI VGPVLTPPQE HPKRPVLGLE CPQSEGPRQS MGHEIKSELL MGGCSWIRGK IQCDRVQVRR PGFSSQL

Purification: SDS-PAGE

Pureté: > 90 %

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Stock: -80 °C,4 °C,-20 °C

Stockage commentaire: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Détail du antigène

Antigène: SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))

Autre désignation: SMUG1 (SMUG1 Produits)

Synonymes: SMUG1 Protein, xsmug1 Protein, 1200013B09Rik Protein, A930006H09Rik Protein, C85220 Protein, FDG Protein, HMUDG Protein, UNG3 Protein, single-strand-selective monofunctional uracil-DNA glycosylase 1 Protein, single-strand selective monofunctional uracil DNA glycosylase Protein, single-strand-selective monofunctional uracil-DNA glycosylase 1 L homeolog Protein, SMUG1 Protein, smug1 Protein, smuG1 Protein, smuG Protein, LOC5577584 Protein, CpipJ_CPIJ002767 Protein, Smug1 Protein, smug1.L Protein

Sujet: Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.

Poids moléculaire: 35.61 kDa

UniProt: Q53HV7

Pathways: Réparation de l'ADN