CD3 epsilon Protein (CD3E) (AA 23-126) (His tag,AVI tag,Biotin)

N° du produit ABIN5954965

Détail du produit

Antigène: CD3 epsilon (CD3E)

Type de proteíne: Recombinant

Attributs du protein: AA 23-126

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette CD3 epsilon protéine est marqué à la His tag,AVI tag,Biotin.

Séquence: AA 23-126

Specificité: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Pureté: >90 % as determined by SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Commentaires:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Référence

Knowling, Clark, Sjuts, Abdiche: "Direct Comparison of Label-Free Biosensor Binding Kinetics Obtained on the Biacore 8K and the Carterra LSA." dans: SLAS discovery : advancing life sciences R & D, Vol. 25, Issue 9, pp. 977-984, (2020) (PubMed).

Détail du antigène

Antigène: CD3 epsilon (CD3E)

Autre désignation: CD3 epsilon (CD3E Produits)

Synonymes: cd3e Protein, CD3E Protein, t3e Protein, tcre Protein, AI504783 Protein, CD3 Protein, CD3epsilon Protein, T3e Protein, T3E Protein, TCRE Protein, CD3e molecule Protein, CD3 antigen, epsilon polypeptide Protein, cd3e Protein, CD3E Protein, Cd3e Protein

Sujet: CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.

Poids moléculaire: 15.5 kDa

NCBI Accession: NP_000724

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway