ITGAV/ITGB5 Protein (AA 31-992) (His tag,AVI tag,Biotin)

N° du produit ABIN5955012

Détail du produit

Antigène: ITGAV/ITGB5 (Integrin alpha V / beta 5 (ITGAV/ITGB5))

Type de proteíne: Recombinant

Attributs du protein: AA 31-992

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette ITGAV/ITGB5 protéine est marqué à la His tag,AVI tag,Biotin.

Séquence: AA 31-992

Specificité: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Pureté: >95 % as determined by SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Commentaires:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 50 mM Tris, 150 mM NaCl, pH 7.5

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Détail du antigène

Antigène: ITGAV/ITGB5 (Integrin alpha V / beta 5 (ITGAV/ITGB5))

Autre désignation: Integrin alpha V beta 5 (ITGAV/ITGB5 Produits)

Sujet: Integrin alpha V beta 5 (ITGAV & ITGB5) is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium. ITGAV & ITGB5 binds ligands containing an RGD motif, notably vitronectin. Growth factors that increase PKC activity, such as VEGF or TGF alpha, promote ITGAV & ITGB5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF alpha. An inhibitor of both down regulates tumor angiogenesis. During lung inflammation, up regulation of ITGAV & ITGB5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF beta from latency.

Poids moléculaire: 114.7 kDa (ITGAV) & 81.9 kDa (ITGB5)