IL-1 beta Protein (AA 117-269) (His tag)

N° du produit ABIN6239741

Détail du produit

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Activité biologique: Active

Attributs du protein: AA 117-269

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette IL-1 beta protéine est marqué à la His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 95 %

Biological Activity Comment: Interleukin-1 beta belongs to the interleukin 1 cytokine family, which plays a critical role in inflammation, immunity, antiviral responses, and a variety of diseases. It has been reported that IL-1β-induced IL-6 production is mediated by both PI3K and IRAK4 in A549 cells. To test the bioactivity of IL-1β, A549 cells were seeded into 24-well plate at a density of 1x105 cells/mL, and allowed to attach overnight before treated with or without certain concentrations (1ng/mL, 10ng/mL) of IL1-β for 4h and IL-6 levels in the cell supernatant were determined by ELISA. IL-6 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL1-β, the data was shown in Table 1 and Figure 1. Sample O.D. value Corrected Concentration of IL-6(cell supernatant of A549 cells) (ng/mL) stimulated with IL-1β(1ng/mL) 1.04 0.975 61.71 stimulated with IL-1β(10ng/mL) 0.966 0.901 56.77 unstimulated 0.309 0.244 12.44 Table 1. IL-6 levels in the cell supernatant of A549 cells up-regulated by IL1-β 80 60 40 20 0 0 1 10 rh IL-1β (ng/ml) Figure 1. IL-6 levels in the cell supernatant of A549 cells up-regulated by IL1-β.

Information d'application

Indications d'application: Isoelectric Point: 5.9

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Autre désignation: Interleukin 1 Beta (IL1b) (IL1B Produits)

Synonymes: IL-1 Protein, IL1-BETA Protein, IL1F2 Protein, IL-1BETA Protein, IL1beta Protein, il1-b Protein, zgc:111873 Protein, IL-1B Protein, IL-1beta Protein, Il-1b Protein, IL1B Protein, IL-1 beta Protein, IL-1b Protein, interleukin 1 beta Protein, interleukin 1, beta Protein, IL1B Protein, il1b Protein, Il1b Protein

Sujet: Alternative Names: IL1-B, IL1-Beta, IL1F2, IL-1β, Interleukin-1 Family Member 2, Catabolin

Poids moléculaire: 22kDa

UniProt: P01584

Pathways: Signalisation NF-kappaB, Interferon-gamma Pathway, Signalisation TLR, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome