IL-11 Protein (AA 22-199) (His tag)

N° du produit ABIN6239760

Détail du produit

Antigène: IL-11 (IL11) (Interleukin 11 (IL11))

Activité biologique: Active

Attributs du protein: AA 22-199

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette IL-11 protéine est marqué à la His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 90 %

Biological Activity Comment: IL11 (Interleukin-11) is a multifunctional cytokine first isolated from bone marrow-derived stromal cells. It stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Besides, IL11 is reported to induce the proliferation of human T-cells, thus, a proliferation assay was conducted to detect the bioactivity of human recombinant IL11 using Jurkat cells. Briefly, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL11. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Cell proliferation of Jurkat cells after incubation with IL11 for 72h observed by inverted microscope was shown in Figure 1. The CCK-8 data was shown in Figure 2. It was obvious that IL11 significantly promoted cell proliferation of Jurkat cells.

Information d'application

Indications d'application: Isoelectric Point: 11.3

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: IL-11 (IL11) (Interleukin 11 (IL11))

Abstract: IL11 Produits

Synonymes: IL11 Protein, AGIF Protein, IL-11 Protein, Il-11 Protein, interleukin 11 Protein, IL11 Protein, Il11 Protein

Sujet: Alternative Names: AGIF, Adipogenesis Inhibitory Factor, Oprelvekin

Poids moléculaire: 27kDa

UniProt: P20809

Pathways: Signalistation JAK/STAT, Negative Regulation of Hormone Secretion