IL-11 Protein (AA 22-199) (His tag)
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- Antigène Voir toutes IL-11 (IL11) Protéines
- IL-11 (IL11) (Interleukin 11 (IL11))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 22-199
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Origine
- Humain
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette IL-11 protéine est marqué à la His tag.
- Fonction
- Human IL-11 Protein
- Séquence
- Pro22-Leu199
- Attributs du produit
- Recombinant Human IL-11 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Pro22-Leu199.
- Pureté
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-11, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-11 R alpha, hFc Tag with the EC50 of 18.6ng/ml determined by ELISA. The affinity constant of 1.86 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- IL-11 (IL11) (Interleukin 11 (IL11))
- Autre désignation
- IL-11 (IL11 Produits)
- Synonymes
- IL11 Protein, AGIF Protein, IL-11 Protein, Il-11 Protein, interleukin 11 Protein, IL11 Protein, Il11 Protein
- Sujet
- Abstract Interleukin-11 (IL-11) is a pleiotropic cytokine that belongs to gp130 family. IL-11 and its receptor, IL-11Ra, are expressed in human cancers,human cancer cells expressed a functional IL-11Ra subunit, which triggered signal transduction either by exogenous recombinant human IL-11 or by autocrine production of IL-11 in cells cultured under hypoxic conditions.
- Poids moléculaire
- 20.24 kDa. Due to glycosylation, the protein migrates to 21-25 kDa based on Tris-Bis PAGE result.
- Pathways
- Signalistation JAK/STAT, Negative Regulation of Hormone Secretion
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