IL-11 Protein (AA 22-199) (His tag)

N° du produit ABIN7274885

Détail du produit

Antigène: IL-11 (IL11) (Interleukin 11 (IL11))

Type de proteíne: Recombinant

Attributs du protein: AA 22-199

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette IL-11 protéine est marqué à la His tag.

Fonction: Human IL-11 Protein

Séquence: Pro22-Leu199

Attributs du produit: Recombinant Human IL-11 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Pro22-Leu199.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-11, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-11 R alpha, hFc Tag with the EC50 of 18.6ng/ml determined by ELISA. The affinity constant of 1.86 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: IL-11 (IL11) (Interleukin 11 (IL11))

Autre désignation: IL-11 (IL11 Produits)

Synonymes: IL11 Protein, AGIF Protein, IL-11 Protein, Il-11 Protein, interleukin 11 Protein, IL11 Protein, Il11 Protein

Sujet: Abstract Interleukin-11 (IL-11) is a pleiotropic cytokine that belongs to gp130 family. IL-11 and its receptor, IL-11Ra, are expressed in human cancers,human cancer cells expressed a functional IL-11Ra subunit, which triggered signal transduction either by exogenous recombinant human IL-11 or by autocrine production of IL-11 in cells cultured under hypoxic conditions.

Poids moléculaire: 20.24 kDa. Due to glycosylation, the protein migrates to 21-25 kDa based on Tris-Bis PAGE result.

Pathways: Signalistation JAK/STAT, Negative Regulation of Hormone Secretion