IL-18 Protein (AA 36-192) (His tag)
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- Antigène Voir toutes IL-18 (IL18) Protéines
- IL-18 (IL18) (Interleukin 18 (IL18))
- Activité biologique
- Active
- Attributs du protein
- AA 36-192
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Origine
- Souris
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette IL-18 protéine est marqué à la His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Attributs du produit
- Tag location: N-terminal His Tag
- Pureté
- > 90 %
- Biological Activity Comment
- IL18 (interleukin 18) is a cytokine that shares similarities in structure with IL1, and is produced by macrophages and other cells. IL18 has been reported to stimulate T cells proliferation and NK cell activity, besides, murine IL18 has been found to enchance the production of INFγ in spleen cells in vitro. Thus, a stimulation assay was conducted to detect the activity of IL18 using murine spleen cells. Briefly, murine spleen cells were seeded into wells of 6-well plates at a density of 2x106 cells/mL in RPMI-1640 with the addition of various concentrations of IL18. After incubation for 48 hours, the concentration of INFγ in the cell supernatant was detected using an ELISA kit. INFγ levels in the cell supernatant of spleen cells increased significantly after stimulated with IL18, the data was shown in Figure 1 IFNγ concentration in the cell supernatant of murine spleen cells up-regulated by IL18
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- Discover our top product IL18 Protéine
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Interleukin 18 (IL18) protein
IL18 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Interleukin 18 (IL18) (Active) proteinIL18 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 18 (IL18) (AA 37-193) protein (Biotin)IL18 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Interleukin 18 (IL18) (AA 37-193) protein (His-IF2DI Tag)IL18 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interleukin 18 (IL18) protein (Fc Tag)IL18 Origine: Humain Hôte: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 18 (IL18) (AA 1-193) protein (GST tag)IL18 Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 18 (IL18) protein (GST tag)IL18 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant > 85 % as determined by reducing SDS-PAGE.
Interleukin 18 (IL18) protein (His tag)IL18 Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE
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- Indications d'application
- Isoelectric Point: 5
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Agent conservateur
- Dithiothreitol (DTT), Other preservative, ProClin
- Précaution d'utilisation
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Antigène
- IL-18 (IL18) (Interleukin 18 (IL18))
- Abstract
- IL18 Produits
- Synonymes
- Igif Protein, Il-18 Protein, IL-18 Protein, IGIF Protein, ChIL-18 Protein, IL-1g Protein, IL1F4 Protein, IL18 Protein, interleukin 18 Protein, Il18 Protein, IL18 Protein
- Sujet
- Alternative Names: IGIF, IL-1g, IL1F4, IL1-F4, Interferon-Gamma-Inducing Factor, Interleukin-1 Family Member 4, Iboctadekin, Interleukin-1 Gamma
- Poids moléculaire
- 19kDa
- UniProt
- P70380
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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