MGLL Protein (AA 1-313) (His tag)

N° du produit ABIN6387881

Détail du produit

Antigène: MGLL (Monoglyceride Lipase (MGLL))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-313

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette MGLL protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MTTSASSHLN KGIKQVYMSL PQGEKVQAMY IWIDGTGEGL RCKTRTLDSE PKCVEELPEW NFDGSSTLQS EGSNSDMYLV PAAMFRDPFR KDPNKLVLCE VFKYNRRPAE TNLRHTCKRI MDMVSNQHPW FGMEQEYTLM GTDGHPFGWP SNGFPGPQGP YYCGVGADRA YGRDIVEAHY RACLYAGVKI AGTNAEVMPA QWEFQIGPCE GISMGDHLWV ARFILHRVCE DFGVIATFDP KPIPGNWNGA GCHTNFSTKA MREENGLKYI EEAIEKLSKR HQYHIRAYDP KGGLDNARRL TGFHETSNIN DFSAGVANRS ASIRIPRTVG QEKKGYFEDR RPSANCDPFS VTEALIRTCL LNETGDEPFQ YKN

Pureté: > 90 % by SDS - PAGE

Biological Activity Comment: Specific activity is >2,800 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0 pmole of L-glutamate to L-glutamine per miunte at pH 7.5 at 37C in coupled system with PK/LDH.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 20 % glycerol, 5  mM DTT, 200  mM NaCl

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: MGLL (Monoglyceride Lipase (MGLL))

Autre désignation: MGLL (MGLL Produits)

Synonymes: cb909 Protein, wu:fc66g01 Protein, zgc:56561 Protein, MGC84195 Protein, MGLL Protein, mgl Protein, hu-k5 Protein, mgll Protein, HU-K5 Protein, HUK5 Protein, MAGL Protein, MGL Protein, AA589436 Protein, Magl Protein, Mgl Protein, monoglyceride lipase Protein, monoglyceride lipase L homeolog Protein, 13L protein Protein, Monoglyceride lipase Protein, mgll Protein, MGLL Protein, mgll.L Protein, 13L Protein, YMTVg13L Protein, EHI_044210 Protein, EHI_045330 Protein, TATV_DAH68_039 Protein, Mgll Protein

Sujet: Glutamine synthetase (GLUL), which is therefore able to regulate intracellular concentrations of glutamate. GLUL catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling. GLUL is essential for proliferation of fetal skin fibroblasts and plays an important role in controlling body  pH by removing ammonia from circulation. Mutations in GLUL are associated with congenital glutamine deficiency. Recombinant GLUL protein , fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 44.2kDa (393aa), confirmed by MALDI-TOF

NCBI Accession: NP_001028216

UniProt: P15104

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Lipid Metabolism