SARS-CoV-2 Spike Protein (P.1 - gamma) (His tag)

N° du produit ABIN7013280

Détail du produit

Antigène: SARS-CoV-2 Spike

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: P.1 - gamma

Origine: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Gamma

Source: HEK-293 Cells

Purification/Conjugué: Cette SARS-CoV-2 Spike protéine est marqué à la His tag.

Séquence: AA 16-1213

Specificité: SARS-CoV-2 S protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F), His Tag (MALS verified)

Attributs du produit: SARS-CoV-2 S protein, His Tag is the ectodomain of SARS-CoV-2 S protein that contains AA Val 16 - Pro 1213 (Accession # QHD43416.1) and mutations, which have become increasingly common in SARS-CoV-2 viruses from around the world. The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. L18F/ T20N/ P26S/ D138Y/ R190S/ K417T/ E484K/ N501Y/ D614G/ H655Y/ T1027I/ V1176F mutations were identified in the SARS-CoV-2 variant (known as variant P.1) which emerged in the Brazil.

Pureté: >95 % as determined by SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Indications d'application: MALS verified

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS

Stock: -20 °C

Détail du antigène

Antigène: SARS-CoV-2 Spike

Autre désignation: SARS-CoV-2 S protein (SARS-CoV-2 Spike Produits)

Synonymes: E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein

Classe de substances: Viral Protein

Sujet: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Poids moléculaire: 138.1 kDa