SARS-CoV-2 Spike Protein (B.1.621 - mu, Trimer) (His tag)
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- Antigène Voir toutes SARS-CoV-2 Spike Protéines
- SARS-CoV-2 Spike
- Type de proteíne
- Recombinant
- Attributs du protein
- B.1.621 - mu, Trimer
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Origine
- SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Mu
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette SARS-CoV-2 Spike protéine est marqué à la His tag.
- Fonction
- SARS-CoV-2 Spike Trimer (T95I, Y144S, Y145N, R346K, E484K, N501Y, D614G, P681H, D950N), His Tag (MALS verified)
- Séquence
- Val 16 - Pro 1213
- Specificité
- SARS-CoV-2 Spike Trimer (T95I, Y144S, Y145N, R346K, E484K, N501Y, D614G, P681H, D950N)
- Attributs du produit
- SARS-CoV-2 Spike Trimer, His Tag (SPN-C52Ha) is the ectodomain of SARS-CoV-2 spike protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The mutations T95I, Y144S, Y145N, R346K, E484K, N501Y, D614G, P681H, D950N were identified in the SARS-CoV-2 Mu variant (Pango lineage: B.1.621). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
- Pureté
- >95 % as determined by SDS-PAGE.
- niveau d'endotoxine
- Less than 1.0 EU per μg by the LAL method.
- Classe de qualité
- MALS verified
- Top Product
- Discover our top product SARS-CoV-2 Spike Protéine
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- Indications d'application
- This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 138 kDa. The protein migrates as kDa under reducing (R) condition due to glycosylation.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- PBS
- Stock
- -20 °C
- Stockage commentaire
- -20°C
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- Antigène
- SARS-CoV-2 Spike
- Abstract
- SARS-CoV-2 Spike Produits
- Synonymes
- E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
- Sujet
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Synonyms: Spike,S protein,Spike glycoprotein,S glycoprotein,
Description: It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. - Poids moléculaire
- 138 kDa
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