CHEK1 Protein (AA 1-476) (His-GST)

N° du produit ABIN7320012

Cette protéine Recombinant CHEK1 est exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour CHEK1 Protein (AA 1-476) (His-GST) (ABIN7320012)

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Baculovirus infected Insect Cells

Pureté: > 90 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 1-476

Purification/Conjugué: Cette CHEK1 protéine est marqué à la His-GST.

Fonction: Recombinant Mouse CHK1/CHEK1 Protein (His & GST Tag)

Séquence: Met1-Thr476

Attributs du produit: A DNA sequence encoding the mouse CHEK1 (O35280-1) (Met1-Thr476) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Kinase activity untested

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.5, 3 mM DTT, 10 % glycerol

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Autre désignation: CHK1/CHEK1

Sujet: C85740,Chk1,rad27,CHK1 / CHEK1 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily. It is a member of checkpoint kinases (Chks). Chks Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle. There are two subtypes of chks that have so far been identified, CHK1 / CHEK1 and Chk2. They are essential components to delay cell cycle progression in normal and damaged cells and can act at all three cell cycle checkpoints. Chks are activated by phosphorylation. ATR kinase phosphorylates CHK1 / CHEK1 in response to single strand DNA breaks and ATM kinase phosphorylates Chk2 in response to double strand breaks. Chks phosphorylate Cdc25 phosphatase at Ser216, which leads to Cdc25 sequestration in the cytoplasm. Chks have a role in the physiological stress of hypoxia/reoxygenation. CHK1 / CHEK1 is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. CHK1 / CHEK1 may also negatively regulate cell cycle progression during unperturbed cell cycles.

Poids moléculaire:

Calculated MW: 82.2 kDa

Observed MW: 78 kDa

UniProt: O35280

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire, Réparation de l'ADN