CHEK1 Protein (GST tag)

N° du produit ABIN7317105

Détail du produit

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette CHEK1 protéine est marqué à la GST tag.

Fonction: Recombinant Human CHK1/CHEK1 Protein (GST Tag)(Active)

Séquence: Met 1-Thr476

Attributs du produit: A DNA sequence encoding the human CHK1 (AAM78553.1) (Met1-Thr476) was fused with the GST tag at the N-terminus.

Pureté: > 90 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 32 nmol/min/mg using CHKtide peptide (KKKVSRSGLYRSPSMPENLNRPR) as substrate.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol, 2 mM GSH

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Détail du antigène

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Autre désignation: CHK1/CHEK1 (CHEK1 Produits)

Synonymes: An08g10320 Protein, AO090003000441 Protein, CHK1 Protein, chk1 Protein, C85740 Protein, Chk1 Protein, rad27 Protein, id:ibd2720 Protein, zgc:56093 Protein, CHEK1 Protein, checkpoint kinase 1 Protein, serine/threonine-protein kinase chk1 Protein, serine/threonine-protein kinase Chk1 Protein, CAMK/CAMKL/CHK1 protein kinase Chk1 Protein, checkpoint kinase 1 S homeolog Protein, CHEK1 Protein, ANI_1_2436074 Protein, AOR_1_768154 Protein, PTRG_04183 Protein, SJAG_01680 Protein, PAAG_04978 Protein, MCYG_03290 Protein, VDBG_03742 Protein, chek1.S Protein, Chek1 Protein, chek1 Protein

Sujet:

Background: CHK1 / CHEK1 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily. It is a member of checkpoint kinases (Chks). Chks Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle. There are two subtypes of chks that have so far been identified, CHK1 / CHEK1 and Chk2. They are essential components to delay cell cycle progression in normal and damaged cells and can act at all three cell cycle checkpoints. Chks are activated by phosphorylation. ATR kinase phosphorylates CHK1 / CHEK1 in response to single strand DNA breaks and ATM kinase phosphorylates Chk2 in response to double strand breaks. Chks phosphorylate Cdc25 phosphatase at Ser216, which leads to Cdc25 sequestration in the cytoplasm. Chks have a role in the physiological stress of hypoxia/reoxygenation. CHK1 / CHEK1 is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. CHK1 / CHEK1 may also negatively regulate cell cycle progression during unperturbed cell cycles.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: CHK1

Poids moléculaire: 80.7kDa

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire, Réparation de l'ADN