POLB Protein (His tag)

N° du produit ABIN7318411

Détail du produit

Antigène: POLB (Polymerase (DNA Directed), beta (POLB))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette POLB protéine est marqué à la His tag.

Fonction: Recombinant Human DNA Polymerase β/POLB Protein (His Tag)

Séquence: Ser2-Glu335

Attributs du produit: Recombinant Human DNA Polymerase beta is produced by our E.coli expression system and the target gene encoding Ser2-Glu335 is expressed with a 6His tag at the C-terminus.

Pureté: > 95 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 1 mM DTT, 1 mM EDTA, 50 % Glycerol, pH 7.8.

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Détail du antigène

Antigène: POLB (Polymerase (DNA Directed), beta (POLB))

Autre désignation: DNA Polymerase beta/POLB (POLB Produits)

Synonymes: wu:fa97a05 Protein, zgc:109924 Protein, POLB Protein, DDBDRAFT_0186152 Protein, DDBDRAFT_0215784 Protein, DDBDRAFT_0232376 Protein, DDB_0186152 Protein, DDB_0215784 Protein, DDB_0232376 Protein, A430088C08Rik Protein, polymerase (DNA directed), beta Protein, DNA polymerase beta Protein, DNA polymerase X family protein Protein, polymerase (DNA directed), beta S homeolog Protein, polb Protein, POLB Protein, polB Protein, polb.S Protein, Polb Protein

Sujet:

Background: Human DNA polymerase β is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. It conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase β.

Synonym: DNA Polymerase Beta, POLB

Poids moléculaire: 39.2 kDa

UniProt: P06746

Pathways: Réparation de l'ADN