PPP3R1 Protein (His tag)

N° du produit ABIN7317211

Détail du produit

Antigène: PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette PPP3R1 protéine est marqué à la His tag.

Fonction: Recombinant Human PPP3R1 Protein (His Tag)(Active)

Séquence: Gly2-Val170

Attributs du produit: A DNA sequence encoding the human PPP3R1 (P63098) (Gly2-Val170) was fused with a polyhistide tag at the N-terminus.

Pureté: > 87 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Using the Octet RED System, the affinity constant (Kd) of human PPP3R1-His bound to Human PPIA-His was 6 nM.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 10 % glycerol, 2 mM DTT

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))

Autre désignation: PPP3R1 (PPP3R1 Produits)

Synonymes: si:ch211-274c22.8 Protein, canb Protein, CALNB1 Protein, CNB Protein, CNB1 Protein, CaNB1 Protein, Cnb1 Protein, MCIP1 Protein, protein phosphatase 3, regulatory subunit B, alpha a Protein, protein phosphatase 3 regulatory subunit B, alpha S homeolog Protein, protein phosphatase 3 regulatory subunit B, alpha Protein, protein phosphatase 3, regulatory subunit B, alpha Protein, protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I) Protein, ppp3r1a Protein, ppp3r1.S Protein, PPP3R1 Protein, Ppp3r1 Protein

Sujet:

Background: PPP3R1 belongs to the calcineurin regulatory subunit family. It is a regulatory subunit of calcineurin. Calcineurin is composed of two subunits: calcineurin A (CnA) and calcineurin B (CnB). Dephosphorylation of the nuclear factor of activated T-cells (NF-AT) by Calcineurin is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells. PPP3R1 is a Ser/Thr-specific calcium and calmodulin-dependent protein phosphatase which takes a vital part in the T cell activation pathway. PPP3R1 is involved in protein dephosphorylation, NFAT protein import into nucleus (ortholog) and epithelial to mesenchymal transition (ortholog). It participates in calcineurin signaling pathway, mitogen activated protein kinase signaling pathway. PPP3R1 interacts with (+)-pilocarpine, 2,4-dinitrotoluene and ammonium chloride. It contains four EF-hand domains and four functional calcium-binding sites. PPP3R1 play an improtant role in the T cell activation pathway.

Synonym: Calcineurin Subunit B Type 1, Protein Phosphatase 2B Regulatory Subunit 1, Protein Phosphatase 3 Regulatory Subunit B Alpha Osoform 1, PPP3R1, CNA2, CNB

Poids moléculaire: 21.4 kDa

UniProt: P63098

Pathways: Signalisation RTK, Signalisation WNT, Fc-epsilon Receptor Signaling Pathway, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling