SFRP4 Protein (His tag)
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- Antigène Voir toutes SFRP4 Protéines
- SFRP4 (Secreted Frizzled-Related Protein 4 (SFRP4))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
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Origine
- Humain
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Source
- CHO Cells
- Purification/Conjugué
- Cette SFRP4 protéine est marqué à la His tag.
- Fonction
- Recombinant Human sFRP4 Protein (His Tag)(Active)
- Séquence
- Met 1-VAL346
- Attributs du produit
- A DNA sequence encoding the human SFRP4 (NP_003005.2) (Met1-VAL346) was expressed with a polyhistidine tag at the C-terminus.
- Pureté
- > 95 % as determined by reducing SDS-PAGE.
- niveau d'endotoxine
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T 1/2 2A6 mouse embryonal fibroblast cells.The ED50 for this effect is typically 2-20 μg/mL in the presence of 20 ng/mL of Wnt3a.
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- Discover our top product SFRP4 Protéine
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Antigène
- SFRP4 (Secreted Frizzled-Related Protein 4 (SFRP4))
- Autre désignation
- sFRP4 (SFRP4 Produits)
- Synonymes
- SFRP4 Protein, FRP-4 Protein, FRPHE Protein, sFRP-4 Protein, secreted frizzled related protein 4 Protein, secreted frizzled-related protein 4 Protein, SFRP4 Protein, Sfrp4 Protein
- Sujet
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Background: SFRP family consists of five secreted glycoproteins in humans acting as extracellular signaling ligands. Each is approximately 300 amino acids in length and contains a cysteine-rich domain (CRD) that shares 30-50% sequence homology with the CRD of Frizzled (Fz) receptors, a putative signal sequence, and a conserved hydrophilic carboxy-terminal domain. SFRPs act as soluble modulators of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations. SFRPs are able to bind Wnt proteins and Fz receptors in the extracellular compartment. The interaction between SFRPs and Wnt proteins prevents the latter from binding the Fz receptors. The Wnt pathway plays a key role in embryonic development, cell differentiation and cell proliferation. SFRP4 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins called FZ domain and a NTR domain . Mouse SFRP4 is highly expressed in the ovary, and is localized to granulosa cells of periovulatory follicles and corpora lutea. It plays a critical role in placental development and implantation, and is also an important factor in the development of the decidual fibrinoid zone, and in trophoblast apoptosis.
Synonym: FRP-4;FRPHE;sFRP-4
- Poids moléculaire
- 39 kDa
- NCBI Accession
- NP_003005
- Pathways
- Signalisation WNT
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