AKT3 Protéine

N° du produit ABIN7319999

Détail du produit

Antigène: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Baculovirus infected Insect Cells

Fonction: Recombinant Mouse AKT3 Protein (aa 106-479)(Active)

Séquence: Ala106-Glu479

Attributs du produit: A DNA sequence encoding the AKT3 (Q9WUA6-1) (Ala106-Glu479) was expressed and purified.

Pureté: > 95 % as determined by SDS-PAGE

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 20 nmol/min/mg using synthetic GSK3-derived peptide (R11-SGRARTSSFAEPGGK) as substrate.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Détail du antigène

Antigène: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Autre désignation: AKT3 (AKT3 Produits)

Synonymes: MPPH Protein, PKB-GAMMA Protein, PKBG Protein, PRKBG Protein, RAC-PK-gamma Protein, RAC-gamma Protein, STK-2 Protein, AI851531 Protein, D930002M15Rik Protein, Nmf350 Protein, Pkbg Protein, AKT serine/threonine kinase 3 Protein, thymoma viral proto-oncogene 3 Protein, v-akt murine thymoma viral oncogene homolog 3b Protein, AKT3 Protein, Akt3 Protein, akt3b Protein

Sujet:

Background: v-akt murine thymoma viral oncogene homolog 3 (AKT3), also known as PKB-GAMMA, with AKT1/PKBalpha, AKT2/PKBbeta, are the memerbers of Akt kinase family, share extensive structural similarity and perform common as well as unique functions within cells. The Akt signaling cascade initiates at the cell surface when growth factors or other extracellular stimuli activate phosphoinositide 3-kinase (PI3K). AKT3 was discovered to be the predominant isoform activated in sporadic melanomas. Levels of activity increased during melanoma progression with metastatic melanomas having the highest activity. Although mechanisms of AKT3 activation remain to be fully characterized, overexpression of AKT3 and decreased PTEN activity play important roles in this process. Targeted reduction of AKT3 activity decreased survival of melanoma tumor cells leading to inhibition of tumor development, which may be therapeutically effective for shrinking tumors in melanoma patients. AKT2 and AKT3 play an important role in the viability of human malignant glioma cells. Targeting AKT2 and AKT3 may hold promise for the treatment of patients with gliomas.

Synonym: AI851531,D930002M15Rik,Nmf350

Poids moléculaire: 43.4 kDa

Pathways: Signalisation PI3K-Akt, Signalisation RTK, Signalisation TLR, Hepatitis C, VEGF Signaling