CD80 Protein (CD80) (AA 35-242) (Fc Tag)

N° du produit ABIN7273892

Détail du produit

Antigène: CD80

Type de proteíne: Recombinant

Attributs du protein: AA 35-242

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette CD80 protéine est marqué à la Fc Tag.

Fonction: Human B7-1/CD80 Protein

Séquence: Val35-Asn242

Attributs du produit: Recombinant Human B7-1/CD80 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Val35-Asn242.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human B7-1, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CTLA-4, His Tag with the EC50 of 66ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CD80

Autre désignation: B7-1 (CD80 Produits)

Synonymes: B7 Protein, B7-1 Protein, B7.1 Protein, BB1 Protein, CD28LG Protein, CD28LG1 Protein, LAB7 Protein, B71 Protein, Cd28l Protein, Ly-53 Protein, Ly53 Protein, MIC17 Protein, TSA1 Protein, CD80 molecule Protein, Cd80 molecule Protein, CD80 antigen Protein, CD80 Protein, Cd80 Protein

Sujet: Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.

Poids moléculaire: 50.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints