CD40 Ligand Protein (CD40LG) (Trimer) (His-DYKDDDDK Tag)

N° du produit ABIN7274318

Détail du produit

Antigène: CD40 Ligand (CD40LG)

Type de proteíne: Recombinant

Attributs du protein: Trimer

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette CD40 Ligand protéine est marqué à la His-DYKDDDDK Tag.

Fonction: Human CD40 Ligand/TNFSF5 Trimer Protein

Séquence: Met113-Leu261

Attributs du produit: Recombinant Human CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Met113-Leu261.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD40 Ligand Trimer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 0.42μg/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CD40 Ligand (CD40LG)

Autre désignation: CD40 Ligand (CD40LG Produits)

Synonymes: CD154 Protein, cd40l Protein, CD40L Protein, HIGM1 Protein, IGM Protein, IMD3 Protein, T-BAM Protein, TNFSF5 Protein, TRAP Protein, gp39 Protein, hCD40L Protein, CD40-L Protein, Cd40l Protein, Ly-62 Protein, Ly62 Protein, Tnfsf5 Protein, CD40 ligand Protein, TNF superfamily member 5 Protein, CD40LG Protein, cd40l Protein, Cd40lg Protein

Sujet: CD40 ligand or CD40L, also called CD154,  is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.

Poids moléculaire: 52.4 kDa, Due to glycosylation, the protein migrates to 55 kDa based on Tris-Bis PAGE result.

UniProt: P29965

Pathways: Signalisation NF-kappaB, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints