IL6RA Protein (AA 20-365) (His-Avi Tag)

N° du produit ABIN7275036

Détail du produit

Antigène: IL6RA (Interleukin 6 Receptor, alpha (IL6RA))

Type de proteíne: Recombinant

Attributs du protein: AA 20-365

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette IL6RA protéine est marqué à la His-Avi Tag.

Fonction: Human IL-6 R alpha/CD126 Protein

Séquence: Leu20-Pro365

Attributs du produit: Recombinant Human IL-6 R alpha/CD126 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu20-Pro365.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-6, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-6 R alpha, His Tag with the EC50 of 0.25μg/ml determined by ELISA. The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: IL6RA (Interleukin 6 Receptor, alpha (IL6RA))

Autre désignation: IL-6R alpha (IL6RA Produits)

Synonymes: IL6R1 Protein, Il6ra Protein, CD126 Protein, IL-6R-1 Protein, IL-6RA Protein, IL6Q Protein, IL6RA Protein, IL6RQ Protein, gp80 Protein, IL-6R Protein, Il6r Protein, interleukin 6 receptor Protein, interleukin 6 receptor, alpha Protein, Il6r Protein, IL6R Protein, Il6ra Protein

Sujet: The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6R alpha) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6R alpha and gp130 have been cloned, sequenced, and expressed.

Poids moléculaire: 41.4 kDa. Due to glycosylation, the protein migrates to 65-78 kDa based on Tris-Bis PAGE result.

Pathways: Signalistation JAK/STAT, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints