IL6RA Protein (His-Avi Tag,Biotin)

N° du produit ABIN7275037

Détail du produit

Antigène: IL6RA (Interleukin 6 Receptor, alpha (IL6RA))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette IL6RA protéine est marqué à la His-Avi Tag,Biotin.

Séquence: Leu20-Pro365

Pureté: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-6, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-6 R alpha, His Tag with the EC50 of 0.54μg/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Stock: 4 °C,-80 °C

Stockage commentaire: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: IL6RA (Interleukin 6 Receptor, alpha (IL6RA))

Autre désignation: IL-6R alpha (IL6RA Produits)

Synonymes: IL6R1 Protein, Il6ra Protein, CD126 Protein, IL-6R-1 Protein, IL-6RA Protein, IL6Q Protein, IL6RA Protein, IL6RQ Protein, gp80 Protein, IL-6R Protein, Il6r Protein, interleukin 6 receptor Protein, interleukin 6 receptor, alpha Protein, Il6r Protein, IL6R Protein, Il6ra Protein

Sujet: IL-6R subunit alpha, IL-6R-alpha, IL-6RA, IL-6R 1, IL6R, CD126, IL6RQ, gp80,The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6R alpha) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6R alpha and gp130 have been cloned, sequenced, and expressed.

Poids moléculaire: 41.4 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

Pathways: Signalistation JAK/STAT, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints