1.Measured by its binding ability in a functional ELISA. Immobilized Human BMP2 at 4 μg/mL (100 μL/well) can bind Human BMPRIB with a linear range of 20-338 ng/mL.|2.Measured by its ability to inhibit rhBMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED50 for this effect is 0.6-2.3 μg/mL in the presence of 30 ng/mL of rhBMP-4.
Crystallography grade
BMPR1B
Origine: Souris
Hôte: Cellules d'insectes
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA, Crys
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Buffer
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Stock
-20 °C,-80 °C
Stockage commentaire
Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Antigène
BMPR1B
(Bone Morphogenetic Protein Receptor, Type IB (BMPR1B))