desert Hedgehog Protein (His tag)

N° du produit ABIN7536339

Détail du produit

Antigène: desert Hedgehog (DHH) (Desert Hedgehog (DHH))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette desert Hedgehog protéine est marqué à la His tag.

Fonction: Recombinant Human HHG-3/DHH Protein

Séquence: CGPGRGPVGR RRYARKQLVP LLYKQFVPGV PERTLGASGP AEGRVARGSE RFRDLVPNYN PDIIFKDEEN SGADRLMTER CKERVNALAI AVMNMWPGVR LRVTEGWDED GHHAQDSLHY EGRALDITTS DRDRNKYGLL ARLAVEAGFD WVYYESRNHV HVSVKADNSL AVRAGG

Specificité: Cys23-Gly198

Stérilité: 0.22 μm filtered

niveau d'endotoxine: <1 EU/μg

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Concentration: 0.4 mg/mL

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS,1 mM DTT, pH 7.4.

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Détail du antigène

Antigène: desert Hedgehog (DHH) (Desert Hedgehog (DHH))

Autre désignation: HHG-3/DHH (DHH Produits)

Synonymes: GDXYM Protein, HHG-3 Protein, SRXY7 Protein, C78960 Protein, Xchh Protein, chh Protein, chh-a Protein, dhh Protein, dhh-1 Protein, dhh-A Protein, hh4 Protein, x-chh Protein, desert hedgehog Protein, desert hedgehog L homeolog Protein, desert hedgehog S homeolog Protein, DHH Protein, dhh Protein, Dhh Protein, dhh.L Protein, dhh.S Protein

Classe de substances: Species

Sujet:

Description: Desert Hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Desert Hedgehog is a secreted, 45 kDa, 373 amino acid (aa) protein that undergoes autocatalytic cleavage between Gly198 and Cys199 catalyzed by the C-terminal domain, which releases the N-terminal domain with a concominant attachment of cholesterol at its new C-terminus. In addition to the C-terminally attached cholesterol, a fatty acid acyl chain is esterified to the N-terminal cysteine (aa 23) via an amide linkage. The 19 kDa N-terminal signaling domain is membrane associated due to its double lipid modifications. Its binding to Patched receptors results in the loss of Patched repression of Smoothened signaling (1 - 4). Dhh binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip) (5). Within the N-terminal domain, human Dhh shares 97 % aa sequence identity with mouse and rat Dhh. It shares approximately 75 % aa seqeuence identity with human Indian (Ihh) and Sonic Hedgehog (Shh) (5). Dhh is produced by Sertoli cells and is required for testis development and spermatogenesis (6, 7). It induces steroidogenic factor 1, which is instrumental in promoting Leydig cell differentiation (8, 9).

Name: GDMN, GDXYM, HHG-3, SRXY7,HHG-3,DHH

ID gène: 50846

UniProt: O43323

Pathways: Signalisation Hedgehog