Leptin Receptor Protein (LEPR) (Fc Tag,His tag)

N° du produit ABIN7535076

Détail du produit

Antigène: Leptin Receptor (LEPR)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette Leptin Receptor protéine est marqué à la Fc Tag,His tag.

Fonction: Active Recombinant Human Leptin receptor/LEP-R/CD295 Protein

Séquence: TAFNLSYPIT PWRFKLSCMP PNSTYDYFLL PAGLSKNTSN SNGHYETAVE PKFNSSGTHF SNLSKTTFHC CFRSEQDRNC SLCADNIEGK TFVSTVNSLV FQQIDANWNI QCWLKGDLKL FICYVESLFK NLFRNYNYKV HLLYVLPEVL EDSPLVPQKG SFQMVHCNCS VHECCECLVP VPTAKLNDTL LMCLKITSGG VIFQSPLMSV QPINMVKPDP PLGLHMEITD DGNLKISWSS PPLVPFPLQY QVKYSENSTT VIREADKIVS ATSLLVDSIL PGSSYEVQVR GKRLDGPGIW SDWSTPRVFT TQDVIYFPPK ILTSVGSNVS FHCIYKKENK IVPSKEIVWW MNLAEKIPQS QYDVVSDHVS KVTFFNLNET KPRGKFTYDA VYCCNEHECH HRYAELYVID VNINISCETD GYLTKMTCRW STSTIQSLAE STLQLRYHRS SLYCSDIPSI HPISEPKDCY LQSDGFYECI FQPIFLLSGY TMWIRINHSL GSLDSPPTCV LPDSVVKPLP PSSVKAEITI NIGLLKISWE KPVFPENNLQ FQIRYGLSGK EVQWKMYEVY DAKSKSVSLP VPDLCAVYAV QVRCKRLDGL GYWSNWSNPA YTVVMDIKVP MRGPEFWRII NGDTMKKEKN VTLLWKPLMK NDSLCSVQRY VINHHTSCNG TWSEDVGNHT KFTFLWTEQA HTVTVLAINS IGASVANFNL TFSWPMSKVN IVQSLSAYPL NSSCVIVSWI LSPSDYKLMY FIIEWKNLNE DGEIKWLRIS SSVKKYYIHD HFIPIEKYQF SLYPIFMEGV GKPKIINSFT QDDIEKHQSD

Specificité: Thr20-Asp839

Pureté: > 95 % by SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: < 1.0 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Leptin protein at 2 μg/mL (100 μL/well) can bind Recombinant Human Leptin R/CD295 protein,the EC₅₀ of Leptin R/CD295 is 261.45 ng/mL.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of 50 mM MES, 100 mM NaCl,1 mM DTT, pH 6.5.

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Détail du antigène

Antigène: Leptin Receptor (LEPR)

Autre désignation: Leptin receptor/LEP-R/CD295 (LEPR Produits)

Synonymes: CD295 Protein, LEP-R Protein, LEPRD Protein, OB-R Protein, OBR Protein, obr Protein, cd295 Protein, ob-rb Protein, Fa Protein, LEPROT Protein, Leprb Protein, Modb1 Protein, OB-RGRP Protein, Obr Protein, db Protein, diabetes Protein, obese-like Protein, obl Protein, ObRb Protein, Ob-R Protein, Ob-RM Protein, lepr Protein, leptin receptor Protein, leptin receptor S homeolog Protein, LEPR Protein, lepr Protein, Lepr Protein, lepr.S Protein

Sujet: CD295,LEP-R,LEPRD,OB-R,OBR,LEPR

ID gène: 3953

UniProt: P48357

Pathways: Signalistation JAK/STAT, AMPK Signaling, Feeding Behaviour