TWEAK Protéine

N° du produit ABIN7535623

Détail du produit

Antigène: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Fonction: Active Recombinant Human TNFSF12/TWEAK Protein

Séquence: KGRKTRARRA IAAHYEVHPR PGQDGAQAGV DGTVSGWEEA RINSSSPLRY NRQIGEFIVT RAGLYYLYCQ VHFDEGKAVY LKLDLLVDGV LALRCLEEFS ATAASSLGPQ LRLCQVSGLL ALRPGSSLRI RTLPWAHLKA APFLTYFGLF QVH

Specificité: Lys97-His249(N-Met)

Pureté: > 95 % by SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: <0.1EU/μg

Biological Activity Comment: 1.Measured by its binding ability in a functional ELISA. Immobilized Human TNFSF12 at 2 μg/mL (100 μL/well) can bind Human TNFRSF12A with a linear range of 0.1-2.3 ng/mL.|2.Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED₅₀ for this effect is typically 0.81-3.24 ng/mL.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Stock: -20 °C,-80 °C

Stockage commentaire: Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Détail du antigène

Antigène: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Autre désignation: TNFSF12/TWEAK (TNFSF12 Produits)

Synonymes: APO3L Protein, DR3LG Protein, TWEAK Protein, Apo3l Protein, Dr3l Protein, Dr3lg Protein, Tweak Protein, TNF superfamily member 12 Protein, tumor necrosis factor (ligand) superfamily, member 12 Protein, TNFSF12 Protein, Tnfsf12 Protein

Sujet:

Description: TNFSF12 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. It can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. It is also found that TNFSF12 promotes proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. TNFSF12 also is a weak inducer of apoptosis in some cell types and mediates NF-kappa-B activation.

Name: TNFSF12,APO3L,DR3LG,TNLG4A,TWEAK

ID gène: 8742

UniProt: O43508-1

Pathways: Apoptose