IL10
Origine: Souris
Hôte: Escherichia coli (E. coli)
Recombinant
> 98.0 % as determined by:(a) Analysis by RP-HPLC.(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Active
It is recommended to reconstitute the lyophilized Mouse Interleukin-10 (IL-10) in sterile 18 M-omega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Stock
-20 °C
Larson, Hübner, Torrero, Morris, Brankin, Swierczewski, Davies, Vonakis, Mitre: "Chronic helminth infection reduces basophil responsiveness in an IL-10-dependent manner." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 9, pp. 4188-99, (2012) (PubMed).
Interleukin-10 (IL-10) is produced by murine T-cells following their stimulation by lectins. The main source for B-cell-derived Interleukin-10 (IL-10) in mice is Ly-1 B-cells that express CD5 and CD11. Murine keratinocytes also produce Interleukin-10 (IL-10). In human Interleukin-10 (IL-10) is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones (resembling Th0, Th1, and Th2) after both antigen-specific and polyclonal activation, by B-cell lymphomas, and by mococytes following cell activation by bacterial lipopolysaccharides and mast cells. B-cells lines derived from patients with acquired immunodeficiency syndrome and Burkitt's lymphoma constitutively secerete large quantities of Interleukin-10 (IL-10) into the conditioned medium. The synthesis of Interleukin-10 (IL-10) by monocytes is inhibited by IL-4 and IL-10. Mouse Interleukin-10 (IL-10) produced in E. coli is a single, non-glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18,885 Da. Synonym: rmIL-10, IL-1. Formulation: The protein was lyophilized after extensive dialysis against 20mM PB, pH7.3, 300mM NaCl buffer.