anti-Singe SMO anticorps pour Immunofluorescence

Recommended SMO Antibody

Antigène
Smoothened Homolog (Drosophila) (SMO) Anticorps
  • FZD11
  • Gx
  • SMOH
  • Smoh
  • E130215L21Rik
  • bnb
  • smoothened
  • XSmo
  • smoothened, frizzled class receptor
  • smoothened, frizzled class receptor L homeolog
  • SMO
  • Smo
  • smo.L
  • smo
Épitope
N-Term
20
15
13
12
9
8
8
8
7
4
2
2
1
1
1
1
1
1
1
Reactivité
Roussette (Chauve-souris), Boeuf (Vache), Chien, Hamster, Cheval, Humain, Singe, Souris, Porc, Lapin, Rat (Rattus)
152
42
37
7
4
3
1
1
1
1
1
Hôte
Lapin
92
34
26
Clonalité
Polyclonal
Conjugué
Cet anticorp SMO est non-conjugé
7
6
6
4
4
4
2
2
2
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
105
90
24
21
13
6
2
1
1
Options

Images

N° du produit ABIN1049353
$ 484.00
Plus shipping costs $45.00

General

Antigène Smoothened Homolog (Drosophila) (SMO) Anticorps
Épitope N-Term
(20), (15), (13), (12), (9), (8), (8), (8), (7), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivité Roussette (Chauve-souris), Boeuf (Vache), Chien, Hamster, Cheval, Humain, Singe, Souris, Porc, Lapin, Rat (Rattus)
(152), (42), (37), (7), (4), (3), (1), (1), (1), (1), (1)
Hôte Lapin
(92), (34), (26)
Clonalité
Polyclonal
Conjugué Cet anticorp SMO est non-conjugé
(7), (6), (6), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(105), (90), (24), (21), (13), (6), (2), (1), (1)
Pubmed 1 référence disponible
Fournisseur

Détail du produit anti-SMO anticorps

Détail du antigène SMO Information d'application Stockage References for anti-SMO antibody (ABIN1049353) Images
Marque IHC-plus™
Specificité Human SMO. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Purification Immunoaffinity purified
Immunogène Synthetic 15 amino acid peptide from N-terminal extracellular domain of human SMO. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Platypus (100%), Opossum (93%), Turkey, Chicken, Xenopus (87%), Zebrafish, Seq squirt (80%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Détail du antigène SMO

Détail du produit anti-SMO anticorps Information d'application Stockage References for anti-SMO antibody (ABIN1049353) Images Haut de la page
Antigène
Autre désignation SMO / Smoothened (SMO Antibody Extrait)
Sujet Name/Gene ID: SMO
Subfamily: Frizzled
Family: GPCR

Synonyms: SMO, Frizzled family member 11, FZD11, Gx, Protein Gx, SMOH, Smoothened, Smoothened homolog
ID gène 6608
Pathways Signalisation Hedgehog

Information d'application

Détail du produit anti-SMO anticorps Détail du antigène SMO Stockage References for anti-SMO antibody (ABIN1049353) Images Haut de la page
Indications d'application Approved: ICC, IF, IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.
Commentaires

Target Species of Antibody: Human

Procédure de l'essai The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Stockage

Détail du produit anti-SMO anticorps Détail du antigène SMO Information d'application References for anti-SMO antibody (ABIN1049353) Images Haut de la page
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C,-20 °C
Stockage commentaire Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Date de péremption 12 months

References for anti-SMO antibody (ABIN1049353)

Détail du produit anti-SMO anticorps Détail du antigène SMO Information d'application Stockage Images Haut de la page
Background publications

Corbit, Aanstad, Singla, Norman, Stainier, Reiter: "Vertebrate Smoothened functions at the primary cilium." dans: Nature, Vol. 437, Issue 7061, pp. 1018-21, 2005

Images

Détail du produit anti-SMO anticorps Détail du antigène SMO Information d'application Stockage References for anti-SMO antibody (ABIN1049353) Haut de la page
Images (Fournisseur)
Immunohistochemistry (IHC) image for anti-Smoothened Homolog (Drosophila) (SMO) (N-Term) antibody (ABIN1049353) Human Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
Immunohistochemistry (IHC) image for anti-Smoothened Homolog (Drosophila) (SMO) (N-Term) antibody (ABIN1049353) Human Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)