anti-Souris MAP1B anticorps pour Immunohistochemistry (Frozen Sections)

Recommended MAP1B Antibody (fourni par: Connectez-vous pour afficher )

Antigène
Microtubule-Associated Protein 1B (MAP1B) Anticorps
  • FUTSCH
  • MAP5
  • Mtap1b
  • A230055D22
  • AI843217
  • LC1
  • Mtap-5
  • Mtap5
  • microtubule-associated protein 1B
  • MAP1B
  • Map1b
Reactivité
Boeuf (Vache), Poulet, Chat, Humain, Souris, Rat (Rattus)
77
61
54
18
18
17
12
12
2
2
1
Hôte
Souris
65
18
Clonalité
Monoclonal
Conjugué
Cet anticorp MAP1B est non-conjugé
6
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC), Microarray (MA), Western Blotting (WB)
45
31
28
18
16
15
13
5
5
2
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N° du produit ABIN269612
Produit non disponible pour cette région.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
1 ABIN449248 ICC IHC (fro) IF IHC WB Rabbit C-Term, pThr1265 Connectez-vous pour afficher Polyclonal 7
1 ABIN4332592 ICC IF IHC IHC (fro) WB Biotin Rabbit C-Term, pThr1265 Connectez-vous pour afficher Polyclonal
1 ABIN4332593 ICC IF IHC IHC (fro) WB DyLight 650 Rabbit C-Term, pThr1265 Connectez-vous pour afficher Polyclonal
1 ABIN4332595 ICC IF IHC IHC (fro) WB DyLight 550 Rabbit C-Term, pThr1265 Connectez-vous pour afficher Polyclonal
1 ABIN4332598 ICC IF IHC IHC (fro) FITC Rabbit C-Term, pThr1265 Connectez-vous pour afficher Polyclonal
1 ABIN4332599 ICC IF IHC IHC (fro) WB DyLight 405 Rabbit C-Term, pThr1265 Connectez-vous pour afficher Polyclonal
1 ABIN4332600 ICC IF IHC IHC (fro) WB Alexa Fluor 488 Rabbit C-Term, pThr1265 Connectez-vous pour afficher Polyclonal
1 ABIN4332601 ICC IF IHC IHC (fro) WB Alexa Fluor 405 Rabbit C-Term, pThr1265 Connectez-vous pour afficher Polyclonal
1 ABIN4332602 ICC IF IHC IHC (fro) WB Alexa Fluor 647 Rabbit C-Term, pThr1265 Connectez-vous pour afficher Polyclonal
1 ABIN4332603 ICC IF IHC IHC (fro) WB Alexa Fluor 700 Rabbit C-Term, pThr1265 Connectez-vous pour afficher Polyclonal
1 ABIN4332594 ICC IF IHC IHC (fro) WB DyLight 488 Rabbit C-Term, pThr1265 Connectez-vous pour afficher Polyclonal
1 ABIN4332591 IHC IHC (fro) WB HRP Rabbit C-Term, pThr1265 Connectez-vous pour afficher Polyclonal
1 ABIN1736696 IHC (fro) IF WB Rabbit IgG C-Term, pThr1265 Connectez-vous pour afficher Polyclonal
1 ABIN1736697 IHC (fro) WB Biotin Rabbit IgG C-Term Connectez-vous pour afficher Polyclonal
1 ABIN1736699 IHC (fro) IF WB DyLight 488 Rabbit IgG C-Term Connectez-vous pour afficher Polyclonal
1 ABIN1736700 IHC (fro) WB HRP Rabbit IgG C-Term Connectez-vous pour afficher Polyclonal
1 ABIN1736698 IHC (fro) IF WB DyLight 650 Rabbit IgG C-Term Connectez-vous pour afficher Polyclonal
1 ABIN1736701 IHC (fro) IF WB DyLight 550 Rabbit IgG C-Term Connectez-vous pour afficher Polyclonal

General

Antigène Microtubule-Associated Protein 1B (MAP1B) Anticorps
Reactivité Boeuf (Vache), Poulet, Chat, Humain, Souris, Rat (Rattus)
(77), (61), (54), (18), (18), (17), (12), (12), (2), (2), (1)
Hôte Souris
(65), (18)
Clonalité (Clone)
Monoclonal   ( )
Conjugué Cet anticorp MAP1B est non-conjugé
(6), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC), Microarray (MA), Western Blotting (WB)
(45), (31), (28), (18), (16), (15), (13), (5), (5), (2)
Pubmed 1 référence disponible
Fournisseur Connectez-vous pour afficher

Détail du produit anti-MAP1B anticorps

Détail du antigène MAP1B Information d'application Stockage References for anti-MAP1B antibody (ABIN269612) Images
Specificité The antibody does not cross-react with other MAPs or tubulin.
Purification Unpurified
Immunogène Rat brain microtubule associated proteins.
Clone AA6
Isotype IgG1

Détail du antigène MAP1B

Détail du produit anti-MAP1B anticorps Information d'application Stockage References for anti-MAP1B antibody (ABIN269612) Images Haut de la page
Antigène
Autre désignation MAP1B (MAP1B Antibody Extrait)
Sujet Gene Symbol: MAP1B
Poids moléculaire Theoretical MW: 271 kDa
ID gène 4131
UniProt P15205
Pathways Dynamique des Microtubules, Regulation of Cell Size

Information d'application

Détail du produit anti-MAP1B anticorps Détail du antigène MAP1B Stockage References for anti-MAP1B antibody (ABIN269612) Images Haut de la page
Indications d'application Western Blot 1:500, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, MicroarrayThe observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Commentaires

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocole Protocol specific for MAP1B Antibody IHC protocol for cryostat sections (Protocol generously provided by Dr. Yimin Wang, Tissue Regeneration Therapeutics Inc )
1. Take out the cryostat section slides from -80C. Dry for few minutes at RT.
. Rinse slides with PBS to get rid of OCT. 3 min x 2, shake
. Fix slides with 1:1 methanol:acetone for 3 min
. Wash slides with PBS, 3 min x
. Circle tissue sections with PAP-Pen,
. Incubation with Normal Horse Serum: 5 % in 2 %BSA (in PBS-Tween20) for 20 minutes.
. MAP1B anti human antibody (1:200 in 2 % BSA 0.1 % Tween20). Incubate for 1.5 hours at 37C in a humid chamber.
. Wash slides with PBS-Tween (0.1 %), 5min x2The following steps are for IHC only
. Blocking of endogenous peroxidase: Incubation with 0.3 % H2O2 for 10 minutes.
. Wash PBS-Tween (0.1 %), 3 min x
. Incubation with secondary antibody (1:200 with PBS, anti mouse-bio, in AK-5002 kit) for 45 minutes at 37C in a humid chamber.
. Wash slides with PBS-Tween (0.1 %) 5min x
. Incubate with VECTAINABC reagent solution (bottle C, Cat No: PK6100, contains Avidin-HRP) for 30 minutes at RT in the dark.(For solution C: 10ml PBS + 2 drops of A and 2 drops of B, kept in 4C with dark)Prewarm at RT for 10min before use and drop 2 drops to the sections.
. Wash slides with PBS-Tween (0.1 %) 5min x
. Incubation with peroxidase substrate (Vector Nova Red, SK-4800) until desired stain intensity develops. Usually 1 to 2 minutes.Prepare the substrate immediately before use: can be used for > 1week.- Add 3 drops of Reagent 1 to 5 mL of DDW, mix well- Add 2 drops of Reagent 2 and mix well- Add 2 drops of Reagent 3 and mix well- Add 2 drops of Hydrogen Peroxide Solution and mix well
. Wash slides with tap water for 5 minutes.
. Hematoxylin nuclear staining: (Filter solution when first use, HE staining Jar,2nd one). Stain slides for 5 seconds at room temperature, then carefully rinse for 3 minutes under running cold tap water,
. Mount the slide with cc mount (Sigma, C9368, 30ml)
Restrictions For Research Use only

Stockage

Détail du produit anti-MAP1B anticorps Détail du antigène MAP1B Information d'application References for anti-MAP1B antibody (ABIN269612) Images Haut de la page
Format Liquid
Buffer Ascites
Buffer contains: 15 mM Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid freeze-thaw cycles
Stock -20 °C
Stockage commentaire Store at -20°C. Avoid freeze-thaw cycles.

References for anti-MAP1B antibody (ABIN269612)

Détail du produit anti-MAP1B anticorps Détail du antigène MAP1B Information d'application Stockage Images Haut de la page
Produit citée dans:

Kaneko, Arayatrakoollikit, Yamanaka, Ito, Okiji: "Immunohistochemical and gene expression analysis of stem-cell-associated markers in rat dental pulp." dans: Cell and tissue research, Vol. 351, Issue 3, pp. 425-32, 2013 (Échantillon (espèces): Rat (Rattus)). Détails: Immunohistochemistry

Images

Détail du produit anti-MAP1B anticorps Détail du antigène MAP1B Information d'application Stockage References for anti-MAP1B antibody (ABIN269612) Haut de la page
Supplier Images
Immunohistochemistry (IHC) image for anti-Microtubule-Associated Protein 1B (MAP1B) antibody (ABIN269612) Immunohistochemistry: MAP1B Antibody (AA6) [ABIN269612] - MAP1B expression on human u...
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1B (MAP1B) antibody (ABIN269612) Immunocytochemistry/Immunofluorescence: MAP1B Antibody (AA6) [ABIN269612] - Staining ...